研究のタイプ: 医学/生物学の研究 (experimental study)

[2.45 GHz または8.2 GHzの無線周波数放射にIn Vitroでばく露されたヒトの血液リンパ球における細胞遺伝研究] med./bio.

Cytogenetic studies in human blood lymphocytes exposed in vitro to 2.45 GHz or 8.2 GHz radiofrequency radiation.

掲載誌: Radiat Res 2006; 166 (3): 532-538

この研究は、健康なヒトのボランティアから採取した末梢血サンプルに、生体外で2.45 GHzまたは8.2 GHzのパルス波無線周波RF電磁放射ばく露を与え、その影響を調べた。2つの周波数のRF放射はそれぞれ、正味順電力21 Wまたは60 W、平均電力密度5 mW / cm2または10 mW / cm2、平均比吸収率2.13 W / kgまたは20.71 W / kg、および2時間のばく露中維持された細胞温度は36.9±0.1℃または37.5±0.2℃であった。同じ血液サンプルのアリコート(一定分量)を、RF擬似ばく露または1.5 Gyガンマ線急性ばく露陽性対照)に用いた。培養リンパ球での染色体異常および小核頻度を検査して、細胞遺伝学損傷の程度を評価した。その結果、この実験に用いた条件下では、RF放射ばく露を受けたリンパ球擬似ばく露を受けたリンパ球での損傷のレベルに有意差はなかった;また、8.2 GHz RF放射ばく露中にフィトヘマグルチニンで刺激されたリンパ球刺激されなかったリンパ球における反応に有意差はなかった;対照的に、ガンマ線照射を受けた陽性対照群では、RF放射ばく露群および擬似ばく露群に比べ、損傷の程度が有意に高かった、と報告している。

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研究目的(著者による)

To determine whether radiofrequency causes cytogenetic damage in human blood lymphocytes exposed in vitro.

詳細情報

Aliquots of the same blood samples from three male donors were used as positive controls (PHA-stimuated or to 1.5 Gy gamma radiation exposed cells).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 h
ばく露2: 8.2 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 h

General information

For positive control, samples were exposed to 1.5 Gy gamma radiation (137Cs source) at a dose rate of 1.008 Gy/min.

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Pulse width 10 µs
Duty cycle 10 %
Repetition frequency 10 kHz
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 1.75 m
チャンバの詳細 The temperature in the anechoic room (18 x 9 x 9 ft) was maintained at 37 ± 1°C. The temperature in the incubator box was maintained at 36.9 ± 0.1°C by a flow of warm air through tubing immersed in a water bath and was monitored continuously using a Vitek probe.
ばく露装置の詳細 T-25 tissue culture flasks containing 6 ml of whole blood were placed in an incubator box at a distance of 1.75 m from the opening of the antenna.
Sham exposure A sham exposure was conducted.
Additional information The cells were allowed to settle at the bottom of the culture flasks for 2 h before exposure.
パラメータ
測定量 種別 Method Mass 備考
電力密度 5 mW/cm² mean 測定値 - -
SAR 2.135 W/kg mean 計算値 - ± 0.005 W/kg

ばく露2

主たる特性
周波数 8.2 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Pulse width 8 ns
Duty cycle 0.04 %
Repetition frequency 50 kHz
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 1.46 m
チャンバの詳細 The anechoic room was 20 x 14 x 10 ft. The temperature in the incubator box was maintained at 37.4 ± 0.4°C by a flow of warm water from a compartment underneath and was monitored continuously using a Vitek probe.
ばく露装置の詳細 T-25 tissue culture flasks containing 6 ml of diluted blood were placed in an incubator box at a distance of 1.46 m from the opening of the antenna.
Sham exposure A sham exposure was conducted.
Additional information In a second group, PHA (1%) was added to the diluted blood, and cultures were incubated at 37 ± 1°C for 24 h to stimulate the lymphocytes before exposure.
パラメータ
測定量 種別 Method Mass 備考
電力密度 10 mW/cm² mean 測定値 - -
SAR 20.71 W/kg mean 計算値 - ± 0.08 W/kg

Reference articles

  • Natarajan M et al. (2002): [ヒト単核球のピークパルスマイクロ波(8.2 GHz)ばく露後のNF-kB DNA結合活性]
  • Vijayalaxmi et al. (1997): [イン・ビトロで2450MHz無線周波放射にばく露したヒト血液リンパ球における増殖および細胞遺伝学的研究]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The levels of cytogenetic damage in radiofrequency irradiation exposed and sham-exposed lymphocytes were not significantly different. Also in the response of unstimulated lymphocytes and lymphocytes stimulated with PHA were no signficant differences when exposed to 8.2 GHz radiofrequency irradiation. In contrast, the positive control cells that had been subjected to 1.5 Gy gamma radiation exhibited significantly more cytogenetic damage than radiofrequency irradiation- and sham-exposed lymphocytes.

研究の種別:

研究助成

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