研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットの海馬における長期増強に対する超低周波電磁界ばく露の影響] med./bio.

Effects of exposure to an extremely low frequency electromagnetic field on hippocampal long-term potentiation in rat

掲載誌: Brain Res 2014; 1564: 1-8

この研究は、超低周波電磁界(ELF-EMF)がラット海馬での長期増強LTP)の誘導およびペアパルス比(PPR)を変化させるか否かを、29匹の成獣雄ウィスターラットで実験的に調べた。ラットをELF-EMFばく露群、擬似ばく露群、対照群に分け、ELF-EMF(50Hz、100 μT)を1日2時間、90日間連続で与えた。ばく露終了後、各個体に麻酔下で刺激用の双極電極(貫通枝)と測定用電極歯状回)を植え込み、高周波刺激により海馬にLTPを誘導した。その結果、ELF-EMFばく露群ではLTP誘導が増加した;ばく露群と他の2群の間には、シナプス電位のスロープおよび集合スパイク電位振幅の有意差が見られた;刺激の前後でのPPRには差がなかった、と報告している。

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研究目的(著者による)

To investigate the effects of an extremely low frequency magnetic field exposure on the synaptic plasticity in the hippocampus of rats.

詳細情報

Three groups of rats were examined: 1.) magnetic field exposure (n=10), 2.) sham exposure (n=9) and 3.) cage control (n=10).
At the end of the exposure period, rats were anesthetized and a stimulating electrode and a recording electrode were inserted in the brain of the rats. Stimulation was performed using a stimulation protocol of 400 Hz (10 bursts of 20 stimuli, 0.2 ms duration).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 2 hours/day for 3 month

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 2 hours/day for 3 month
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 solenoid coil with length of 2 m, radius 20 cm and 100 turns/meter; diameter of copper wire 2 mm; adjustable resistance curcuit was connected to the power source to generate electric and magnetic field
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In exposed rats, the excitatory postsynaptic potential slope and the compound action potential were significantly higher in comparison to the sham exposure and the cage control group. No significant differences were observed between the sham exposure and the cage control.
No significant difference in the paired-pulse ratio of the magnetic field exposed rats was observed before and after the stimulation.
The authors conclude that extremely low frequency magnetic fields may change the synaptic plasticity in the hippocampus of rats.

研究の種別:

研究助成

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