研究のタイプ: 医学／生物学の研究 (experimental study)

[正弦波電磁界はインビトロでラットの大腿骨組織の骨形成促進と骨再吸収抑制を起こす] med./bio.

Sinusoidal electromagnetic fields promote bone formation and inhibit bone resorption in rat femoral tissues in vitro

著者: Zhou J, Ma XN, Gao YH, Yan JL, Shi WG, Xian CJ, Chen KM
掲載誌: Electromagn Biol Med 2016; 35 (1): 75-83
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この研究は、インビトロでラット大腿骨組織における骨形成と骨再吸収に対する正弦波電磁界（SEMFs）の影響を調べた。皮質骨と骨梁の培養組織に、SEMFs（50 Hz、1.8mT）のばく露を1日1.5時間、最長12日間与え、ばく露終了後に骨形成と骨再吸収のマーカと関連遺伝子発現を検査した。その結果、SEMFsばく露した皮質骨または骨梁の組織で、アルカリホスファターゼ（ALP）活性の有意な上昇と酒石酸抵抗性酸ホスファターゼ（TRACP）活性の抑制が見られた；また、骨組織のオステリックス（OSX）、インシュリン様増殖因子（IGF-1）、ALPのmRNA発現レベルが有意に上昇した､などの所見を報告している。

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研究目的（著者による）

The effects of exposure of cultured rat femur tissue to a 50 Hz magnetic field on bone formation and resorption and gene expression of associated markers should be investigated.

詳細情報

To examine the major types of bone tissues in a long bone, femoral diaphysis (mainly cortical bone) and metaphysis (containing a large amount of trabecular bone) tissues were extracted from rats, cultured for 48 hours and exposed to the magnetic field.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 1.5 hours/day for up to 12 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 1.5 hours/day for up to 12 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 60-mm culture dishes in a CO2 incubator (5% CO2, 37°C and 100% humidity)
ばく露装置の詳細 6 culture dishes were exposed at a time and placed within the solenoid, which was composed of a core cylinder of acrylic tube (inner diameter: 18 cm; height: 21 cm) and three series-connected coils, which respectively had 24 x 8, 24 x 7 and 24 x 8 turns, with the dimension of the wire being 5 mm x 2 mm; the magnetic field was homogeneous with <5% both horizontally and vertically error
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1.8 mT - 測定値 - -

Reference articles

  • Zhou J et al. (2014): [さまざまな電磁界波形はインビトロでの骨芽細胞の増殖、分化および石灰化にそれぞれ異なる影響を与える]
  • Zhou J et al. (2011): [ラット骨芽細胞の増殖、分化およびミネラル化ポテンシャルに対する、異なる強度の50Hz正弦波電磁界の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

In exposed femoral diaphyseal and metaphyseal tissues, a significant increase in alkaline phosphatase enzyme activity and a significant decrease in tartrate-resistant acid phosphatase enzyme activity compared to the control group was found. Moreover, significantly increased gene expressions of bone formation-related genes osterix, insulin-like growth factor and alkaline phosphatase were observed in both bone tissues after exposure to the magnetic field compared to the control group.
A significantly decreased glucose content and significantly increased lactate content were observed in both bone tissue culture media after exposure to the magnetic field compared to the control group. The gene expressions of bone resorption-related genes tartrate-resistant acid phosphatase, macrophage colony stimulating factor and cathepsin K were significantly decreased in both exposed tissues compared to the control groups.
The authors conclude that exposure of cultured rat femur tissue to a 50 Hz magnetic field might promote bone formation, increase the metabolism and reduce bone resorption.

研究の種別:

研究助成

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