研究のタイプ: 医学/生物学の研究 (experimental study)

[さまざまな電磁界波形はインビトロでの骨芽細胞の増殖、分化および石灰化にそれぞれ異なる影響を与える] med./bio.

Different electromagnetic field waveforms have different effects on proliferation, differentiation and mineralization of osteoblasts in vitro.

掲載誌: Bioelectromagnetics 2014; 35 (1): 30-38

この研究は、さまざまな種類の波形電磁界(EMFs;正弦波、三角波、矩形波、鋸歯状波;全て周波数50Hz、磁界強度1.8 mT)がラット頭蓋冠由来骨芽細胞増殖分化、石灰化に与える影響を比較した。その結果、矩形波分化促進し、正弦波は阻害した;矩形波、鋸歯状波に比べ正弦波と三角波では、アルカリフォスファターゼ(ALP)活性・ALP染色面積・カルシウム沈着・石灰化結節の面積・Runx-2のmRNA発現・オステオプロテゲリンおよびインスリン様成長因子Iが有意に増加した;正弦波に比べ三角波では、Runx-2のmRNA発現を除く全ての評価項目に対する影響が大きかった、と報告している。

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研究目的(著者による)

To study the effects of different waveforms on cell proliferation, differentiation and mineralization of rat osteoblasts.

詳細情報

Four different waveforms and an untreated control were examined: sinusoidal, triangular, rectangular and sawtooth.
Osteoblasts (primary cells) were isolated from skull bones of neonatal rats.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 30 min/day for up to 12 days
ばく露2: 50 Hz
ばく露時間: 30 min/day for up to 12 days
triangular
ばく露3: 50 Hz
ばく露時間: 30 min/day for up to 12 days
ばく露4: 50 Hz
ばく露時間: 30 min/day for up to 12 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 30 min/day for up to 12 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 osteoblasts placed in 60 mm culture dishes inside the solenoid
ばく露装置の詳細 solenoid consisting of three series-connected coils and the core cylinder was an acrylic tube (inner diameter = 18 cm, height = 21 cm); solenoid positioned in an incubator at 37°C
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.8 mT effective value 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 30 min/day for up to 12 days
Additional information triangular
Additional information Signal form: triangular
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.8 mT effective value 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 30 min/day for up to 12 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.8 mT effective value 測定値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sawtooth-shaped
ばく露時間 30 min/day for up to 12 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.8 mT effective value 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Compared to the untreated control, sinusoidal magnetic field exposure significantly inhibited osteoblast proliferation while rectangular magnetic field exposure significantly increased the proliferation.
After 9 and 12 days of exposure, the enzyme activity of the alkaline phosphatase was significantly higher in the sinusoidal and triangular waveform exposed cultures compared to the control cultures. The amount of alkaline phosphatase positive colonies was significantly higher in the sinusoidal, triangular and sawtooth waveform exposed cultures than in the control cultures.
Regarding mineralization, after 9 and 12 days of exposure to sinusoidal and triangular waveforms (and amount of calcium also sawtooth after 12 days), the amount of calcium and the number of calcified nodules were significantly higher than in the control cultures.
In comparison to the control cultures, the gene expression of Runx-2 and Opg was significantly upregulated after 1 day of exposure to a sinusoidal, triangular and sawtooth waveform, whereas the gene expression of Igf-1 was significantly increased after 3 and 4 days.
While rectangular magnetic fields increased the proliferation and did not effect the differentiation of osteoblasts, sinusoidal fields inhibited the proliferation but promoted osteogenic differentiation. The authors conclude that the influence of magnetic fields on proliferation, differentiation and mineralization of rat osteoblasts is dependent on the waveform.

研究の種別:

研究助成

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