研究のタイプ: 医学/生物学の研究 (experimental study)

[金属ナノ粒子を含むまたは含まない細胞外マトリックス構造物中での細胞増殖] med./bio.

In vitro electromagnetic stimulation to enhance cell proliferation in extracellular matrix constructs with and without metallic nanoparticles.

掲載誌: J Biomed Mater Res B Appl Biomater 2015; 103 (8): 1532-1540

この研究は、金のナノ粒子(AuNPs;直径20または100nm)を結合させた非細胞性のブタ組織組織工学的に構成)上にマウス線維芽細胞(L929)を播き、そこに超低周波電磁界(ELF-EMFs:12ガウス、60Hz)を1日2時間、10日間連続ばく露し、L929細胞の増殖へのELF-EMFsの影響を調べた。その結果、ELF-EMF刺激細胞増殖を有意に増強した;AuNPsの存在は、ELF-EMFsの刺激効果を押し上げなかったが、実験3日目から10日目までの増殖速度を上昇させた、と報告している。

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研究目的(著者による)

The effects of an in vitro exposure of murine fibroblasts to a 60 Hz magnetic field in combination with gold nanoparticles on cell growth should be investigated.

詳細情報

Exposed and sham-exposed cells were investigated with or without co-exposure to gold nanoparticles (20 nm and 100 nm diameter) on extracellular matrix structures. The gold nanoparticles were bound covalently to the extracellular matrix structures via crosslinks with the help of a reagent. For the control, only the crosslink reagent was used without gold nanoparticles and it was referred to as the crosslinked group. The study was conducted in view of a possible use in tissue engineering.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 0.5, 1 or 2 hours daily for up to 10 days

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 0.5, 1 or 2 hours daily for up to 10 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cells were incubated in a polyvinyl chloride cell culture chamber within a pair of Helmholtz coils in multi-well cell culture plates containing the extracellular matrix constructs; cell culture chamber maintained a temperature of 37±0.5°C and 5% CO2
ばく露装置の詳細 Helmholtz coils were each constructed of 10 AWG copper magnet wire with an inner diameter of 95.5 cm and a coil height of 6.2 cm; field with <1% deviation in field strength over an area of 25.4 cm in diameter
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.2 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cell populations in magnetic field exposed cultures with and without co-exposure to 20 nm gold nanoparticles appeared to show denser coverage of the matrix surfaces compared to sham-exposed cultures in microscopical analysis. Cell viability was significantly reduced in cultures with 20 nm particles after the first 3 days compared to the crosslinked group, regardless of the concentration and exposure to the magnetic field. In cultures exposed to the magnetic field, as well as in cultures with 100 nm particles alone, a significant higher cell proliferation could be found from day 3-10 compared to sham-exposed cultures and the crosslinked group, respectively. The daily exposure duration (0.5, 1 or 2 h) had no significant effect on cell proliferation.
The authors conclude that an in vitro exposure of murine fibroblasts to a 60 Hz magnetic field as well as to 100 nm gold nanoparticles can enhance cell proliferation. However, a co-exposure does not show any synergistic effects. Further studies with stronger magnetic fields and/or larger nanoparticles could provide other results, though.

研究の種別:

研究助成

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