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The effects of exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on a nanofibrous scaffold on hepatic differentiation should be investigated. A possible therapeutic use for the treatment of hepatic diseases via tissue engineering should be examined.
Cell differentiation was induced by the addition of different growth factors.
Cells were divided into the following groups: 1) cells with growth factors exposed to the magnetic field and cultivated on nanofibrous scaffold, 2) non-exposed cells with growth factors cultivated on nanofibrous scaffold, 3) non-exposed cells with growth factors cultivated on polystyrenes scaffolds, 4) non-exposed cells without growth factors cultivated on nanofibrous scaffold, 5) non-exposed cells without growth factors cultivated on polystyrenes scaffold.
HepG2 cells were used as positive control in the test on the amount of inducible cytochrome P450.
All experiments were performed in triplicates.
ばく露時間: continuous for 28 days
|ばく露時間||continuous for 28 days|
|ばく露装置の詳細||a solenoid iron coil was placed inside an incubator with 5% CO2 and 37°C; the culture flasks were placed over the magnetic field generator|
Morphologically, more polygonal shaped cells, representative for hepatic morphology, were found in group 1 (growth factors, MF, nanofibrous scaffold) than in group 2 (growth factors, no MF, nanofibrous scaffold) and group 3 (growth factors, no MF, polystyrenes scaffold).
Immunofluorescence staining confirmed these results by showing the highest protein expression of hepatocyte specific markers in group 1, a scanty protein expression in groups 2 and 3, and no protein expression in groups without growth factors (groups 4 and 5).
The amounts of albumin and urea were significantly increased in group 1 compared to groups 2 and 3 (groups 4 and 5 did not show any presence of albumin or urea) and likewise, group 1 showed the highest amount of inducible cytochrome P450.
The authors conclude that exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on the nanofibrous scaffold might augment the hepatic differentiation.