研究のタイプ: 医学/生物学の研究 (experimental study)

[低周波磁気力が生体磁気ナノファイバ足場上での間葉系幹細胞の肝分化を増強する] med./bio.

Low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic nanofibrous scaffold.

掲載誌: J Mater Sci Mater Med 2014; 25 (11): 2579-2589

この研究は、末期肝不全治療のための高分子ナノファイバ足場と幹細胞を用いた肝組織工学への低周波磁界の利用を検討した。ポリL乳酸(PLLA)、コラーゲン、高濃度フィブリン血液から生体磁気電界紡糸により製作されたナノファイバ足場上のヒト間葉幹細胞(hMSCs)が、低周波磁界下で肝細胞に形質転換する潜在力を評価した。足場の表面特性、生化学的および生体機械学的パラメータ、生体磁気的ふるまいに関する特徴を記述している。その結果、用いた足場はhMSCsの付着および増殖に適していることが示された;磁界ばく露群の方が対照群に比べ、足場上のhMSCsの肝細胞への形質転換力が高まった、と報告している。

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研究目的(著者による)

The effects of exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on a nanofibrous scaffold on hepatic differentiation should be investigated. A possible therapeutic use for the treatment of hepatic diseases via tissue engineering should be examined.

詳細情報

Cell differentiation was induced by the addition of different growth factors.
Cells were divided into the following groups: 1) cells with growth factors exposed to the magnetic field and cultivated on nanofibrous scaffold, 2) non-exposed cells with growth factors cultivated on nanofibrous scaffold, 3) non-exposed cells with growth factors cultivated on polystyrenes scaffolds, 4) non-exposed cells without growth factors cultivated on nanofibrous scaffold, 5) non-exposed cells without growth factors cultivated on polystyrenes scaffold.
HepG2 cells were used as positive control in the test on the amount of inducible cytochrome P450.
All experiments were performed in triplicates.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 28 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 28 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 culture flasks
ばく露装置の詳細 a solenoid iron coil was placed inside an incubator with 5% CO2 and 37°C; the culture flasks were placed over the magnetic field generator
パラメータ
測定量 種別 Method Mass 備考
磁束密度 60 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Morphologically, more polygonal shaped cells, representative for hepatic morphology, were found in group 1 (growth factors, MF, nanofibrous scaffold) than in group 2 (growth factors, no MF, nanofibrous scaffold) and group 3 (growth factors, no MF, polystyrenes scaffold).
Immunofluorescence staining confirmed these results by showing the highest protein expression of hepatocyte specific markers in group 1, a scanty protein expression in groups 2 and 3, and no protein expression in groups without growth factors (groups 4 and 5).
The amounts of albumin and urea were significantly increased in group 1 compared to groups 2 and 3 (groups 4 and 5 did not show any presence of albumin or urea) and likewise, group 1 showed the highest amount of inducible cytochrome P450.
The authors conclude that exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on the nanofibrous scaffold might augment the hepatic differentiation.

研究の種別:

研究助成

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