研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界はGC-2細胞株のシグナル伝達経路におけるmiRNA介在性の調節機構を変化させる] med./bio.

Extremely low-frequency electromagnetic fields affect the miRNA-mediated regulation of signaling pathways in the GC-2 cell line.

掲載誌: PLoS One 2015; 10 (10): e0139949

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The effects of exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field on the microRNA expression and the possible use of microRNAs as biomarkers for magnetic field exposure should be investigated.

詳細情報

MicroRNAs are small non-coding RNAs, which regulate gene expression at the post-transcriptional level. Mouse spermatocyte-derived cells were investigated to explore the mechanisms of action of possible adverse effects of magnetic fields on the male reproductive system. Cells were divided into the following groups: exposure to a magnetic field of 1) 1 mT, 2) 2 mT and 3) 3 mT. For each group, a separate sham exposure was conducted simultaneously.
Except for microarray experiments, all tests were repeated three times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: intermittent (5 min on/10 min off) for 72 h
ばく露2: 50 Hz
ばく露時間: intermittent (5 min on/10 min off) for 72 h
ばく露3: 50 Hz
ばく露時間: intermittent (5 min on/10 min off) for 72 h

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on/10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
チャンバの詳細 dishes in metal chamber
ばく露装置の詳細 two rectangular waveguides and two four-coil systems (two coils with 56 windings, two coils with 50 windings) generated a vertical magnetic field inside the chamber; system was composed of two identical exposure chambers (one for exposure, the other for simultaneous sham exposure); environmental conditions were constant (37°C, 5% CO2); exposure started after overnight starvation
Sham exposure A sham exposure was conducted.
Additional information the temperature difference between sham exposure and exposure chamber never exceeded 0.3°C
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on/10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on/10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences were found in cell morphology, cell viability, apoptosis and cell cycle distribution between the exposure and respective sham exposure groups.
In the microarrays, 19 microRNAs with a significantly differential expression rate were found in group 1 (1 mT) (7 microRNAs were upregulated, 12 were downregulated) and 36 microRNAs were significantly differentially expressed (9 miRNAs were upregulated, and 27 were downregulated) in group 3 (3 mT) compared to the sham exposure groups. This results were confirmed by the real-time RT-PCR.
The pathway analyses showed that the identified microRNAs may regulate circadian rhythms, cytokine-cytokine receptor interactions and the p53 signaling pathway.
The authors conclude that exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field could alter the microRNA expression and that microRNAs could serve as potential biomarkers for magnetic field exposure.

研究の種別:

研究助成

関連論文