研究のタイプ: 医学/生物学の研究 (experimental study)

[マウス精母細胞由来細胞株GC-2のDNAメチル化およびDNAメチル基転移酵素に対する50 Hz超低周波電磁界の影響] med./bio.

Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.

掲載誌: Biomed Res Int 2015: 237183

この研究は、50 Hz超低周波電磁界(ELF-EMF)の男性生殖系へエピジェニックな影響を調べるイン・ビトロ実験を行った。マウス精母細胞由来細胞株GC-2に50 Hzの ELF-EMF(1 mT、2 mT、3 mT)を断続的(5分間ON、10分間OFF)に72時間ばく露した。その結果、ゲノムDNA全体のメチル化レベルは、1 mTでは擬似ばく露より低く、3 mTでは高かった;同様にDNMT1およびDNMT3bの発現は、1 mTでは擬似ばく露より減少し、3 mTでは増加した;一方、DNMT3a発現への影響は無かった、と報告している。

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研究目的(著者による)

The epigenetic effects of exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field should be investigated by means of DNA methylation and gene expression analysis.

詳細情報

Mouse spermatocyte-derived cells were investigated to explore the mechanisms of action of possible adverse effects of magnetic fields on the male reproductive system.
Cells were divided into 4 groups: exposure to a magnetic field of 1) 1 mT, 2) 2 mT, 3) 3 mT and 4) sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: intermittent (5 min on, 10 min off) for 72 h
ばく露2: 50 Hz
ばく露時間: intermittent (5 min on, 10 min off) for 72 h
ばく露3: 50 Hz
ばく露時間: intermittent (5 min on, 10 min off) for 72 h

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
チャンバの詳細 90 mm petri dishes
ばく露装置の詳細 cell dishes were placed in an incubator (constant 37°C, 5% CO2); the exposure system generated a vertical field via two four-coil systems (two coils with 56 windings, two coils with 50 windings) and two rectangular waveguides placed in a metal chamber
Sham exposure A sham exposure was conducted.
Additional information the temperature difference between sham exposure and exposure never exceeded 0.3°C
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittent (5 min on, 10 min off) for 72 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The global DNA methylation was significantly lower in cells exposed to 1 mT (group 1) compared to the sham exposure group and significantly higher in cells exposed to 2 mT and 3 mT (groups 2 and 3).
In the mRNA and protein expression of DNA methyl-transferases, a trend towards decreased expression rates in groups 1 and 2 and increased expression rates in group 3 in comparison to the sham exposure group was observed.
In the genome wide differential promoter DNA methylation analysis, 296 differentially methylated sites (higher percentage of enhanced methylation) compared to the sham exposure were found in group 1 and 70 differentially methylated sites (higher percentage of decreased methylation) were found in group 3. Comparing the promoter methylation of selected genes with their mRNA expression in both groups, genes with decreased methylation showed a significantly increased mRNA expression and genes with increased methylation showed a significantly decreased mRNA expression.
The genome wide mRNA expression showed 84 differentially expressed genes in group 1 and 324 differentially expressed genes in group 3 compared to the sham exposure group. In both groups, most affected genes belonged to olfactory transduction pathway but different target genes were identified in each group.
The authors conclude that exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field might have epigenetic effects via DNA methylation with consequential altered gene expression, although the consequences for health remain unknown.

研究の種別:

研究助成

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