研究のタイプ: 医学/生物学の研究 (experimental study)

[マウス副腎細胞株において超低周波磁界ばく露はホスホジエステラーゼ活性阻害を介して副腎ステロイド合成を刺激する] med./bio.

Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line.

掲載誌: PLoS One 2016; 11 (4): e0154167-

この研究は、マウス副腎皮質由来Y-1細胞株およびヒト副腎皮質由来H295R細胞株を用い、超低周波磁界(ELF-MF:60Hz)が直接的に副腎ステロイド生成を刺激するか否かを調べた。コイルで構成されたばく露装置および擬似ばく露装置を用いて1-48時間のばく露擬似ばく露)を培養皿に与えた。ばく露中のELF-MF磁界強度は0.25-3mT、ばく露装置および擬似ばく露装置内の背景レベルは静磁界40-50μT、ELF-MF25-30μTであった。その結果、Y-1細胞株では、ELF-MFばく露副腎ステロイド生成およびその関連酵素発現を有意に高まったが、H295R細胞株では影響が弱かった;ELF-MFばく露したY-1細胞株では、ホスホジエステラーゼ活性、細胞内カルシウムイオン濃度の有意な低下、細胞内cAMP濃度、cAMP応答配列結合タンパク質リン酸化有意な上昇が見られた、と報告している。

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研究目的(著者による)

The effects of exposure of mouse and human adrenal cortex-derived cells to a 60 Hz magnetic field on steroid hormone production and the underlying mechanisms of action should be investigated.

詳細情報

Except for one test, all experiments, a magnetic flux density of 1.5 mT was used. Only for the quantification of corticosterone, mouse adrenal cortex-derived cells were exposed to 0.25 mT, 0.5 mT, 1.5 mT and 3 mT.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: for 1 h, 6 h, 24 h or 48 hours
ばく露2: 60 Hz
ばく露時間: for 24 hours
ばく露3: 60 Hz
ばく露時間: for 24 hours
ばく露4: 60 Hz
ばく露時間: for 24 hours

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 for 1 h, 6 h, 24 h or 48 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell culture dishes in incubator
ばく露装置の詳細 the exposure apparatus consisted of a cylindrical plastic (polyvinyl chloride) container (37 cm outer diameter, 34.5 cm inner diameter, 33 cm length) placed vertically with a coil inside; the coil consisted of one primary coil and two secondary coils that were placed on the upper and lower ends of the primary coil; coils were made of enameled wires (2.12 mm diameter); for the primary coil, the wire was wound 150 times around the plastic container; for the two secondary coils, the wire was wound 33 times around every layer to correct the uneven distribution of the magnetic flux density of the primary coil; a plastic incubator (25 cm length, 20 cm width and 10 cm height) containing the culture dishes was placed in the middle of each plastic container; to maintain the temperature of 37 ± 0.5°C and 5% CO2 in the incubator, warm water (40 ± 0.5°C) and 5% CO2 air were supplied in the container; the temperature difference between ELF-MF and sham exposure apparatuses was maintained within ± 0.5°C
Sham exposure A sham exposure was conducted.
Additional information sham exposure was performed simultaneously with exposure using a sham apparatus, which was made using the same materials with the same size, except for the coil
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.25 mT - 測定値 - -

ばく露3

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

ばく露4

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 for 24 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - 測定値 - -

Reference articles

  • Soda A et al. (2008): [骨芽細胞様細胞におけるシグナル伝達経路に関する細胞コラーゲンに対する超低周波電磁界へのばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to the magnetic field was found to significantly increase the secretion of corticosterone and aldosterone and the gene expression and/or protein expression of Cyp11a1 and Cyp11b2 in mouse Y-1 cells compared to the control group. It was also found that only a magnetic flux density of 0.5 mT or more could induce a significant increase in corticosterone secretion in exposed Y-1 cells compared to the control group.
Exposed Y-1 cells showed significant decreases in phosphodiesterase enzyme activity and intracellular calcium concentration and significant increases in cAMP concentration and CREB phosphorylation compared to cells of the control group. The increase in cAMP concentration was not inhibited by treatment with NF449, suggesting that it did not result from an activation of G protein-coupled receptors.
No effects were found in human adrenal cortex-derived cells.
The authors conclude that exposure of mouse adrenal cortex-derived cells to a 60 Hz magnetic field might stimulate the steroid hormone production via an increase in intracellular cAMP caused by the inhibition of phosphodiesterase enzyme activity.

研究の種別:

研究助成

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