研究のタイプ: 医学/生物学の研究 (experimental study, review/survey)

[イン・ビボでの骨形成への電磁刺激の影響研究に決定的意味を持つ骨芽細胞モデルの選択] med./bio.

Choice of osteoblast model critical for studying the effects of electromagnetic stimulation on osteogenesis in vitro.

掲載誌: Electromagn Biol Med 2016; 35 (4): 353-364

この研究は、電磁界EMF)の骨形成促進作用についての異なる研究結果(骨芽細胞細胞の増殖促進か、または対照的に分化誘導およびミネラル化増強か)が得られた原因は方法論の違いにあると考えて、選択された骨芽細胞モデルが電磁刺激の結果に与える影響を調べた。その結果、表現型が未成熟の細胞(特に低い播種密度での)では、EMFにより増殖が大きくなり、高密度の成熟細胞では分化およびミネラル化が早めに開始される傾向が見られた、と報告している。

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研究目的(著者による)

The effects of exposure of two different osteoblast cell lines to a 50 Hz magnetic field on proliferation and osteogenic differentiation should be investigated.

詳細情報

The study comprised a survey and an experimental part. Based on the survey, the authors hypothesized, that disparities between studies regarding the effects of electro-magnetic field exposure on osteoblasts result from the chosen cell line and depend on the cells' level of maturity. Hence, in the present study, the more immature MC3T3-E1 cells and the relatively mature SaOS-2 cells were investigated.
The following exposure groups were used: MC3T3-E1 cells seeded at 1) low seeding density, 2) recommended seeding density and 3) high seeding density and SaOS-2 cells seeded at 4) low seeding density, 5) recommended seeding density and 6) high seeding density. For each exposure group, a separate control group was used. Moreover, cells were tested with and without osteogenic differentiation medium.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 30 minutes/day

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 minutes/day
ばく露装置
ばく露の発生源/構造
チャンバの詳細 coils in incubator
ばく露装置の詳細 225-round Helmholtz coils with radius of 10 cm
Additional information control samples were kept in an identical separate incubator
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cell proliferation and cell viability were significantly increased in exposed MC3T3-E1 cells at all points in time and all plating densities (groups 1-3) compared to the control groups, while they were significantly decreased in SaOS-2 cells (groups 4-6) compared to the control groups. The alkaline phosphatase enzyme activity was significantly increased in all exposure groups and at all points in time compared to the control groups. However, base line levels in SaOS-2 cells were higher than in MC3T3-E1 cells and SaOS-2 cells showed a stronger reaction towards the magnetic field.
Exposure to the magnetic field in combination with differentiation medium resulted in a significant increase in mineralization in all groups, with the most distinct effect in SaOS-2 cells.
The authors conclude that exposure of osteoblasts to a 50 Hz magnetic field might increase proliferation or osteogenic differentiation to different extents depending on the cell line. Different reactions of both cell lines indicated that EMF-induced effects on proliferation and differentiation could depend on the maturation stage of the cells.

研究の種別:

研究助成

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