研究のタイプ: 医学/生物学の研究 (experimental study)

[ベニコウジカビの色素産生に対する低周波磁界の影響] med./bio.

Effect of low-frequency magnetic field on formation of pigments of Monascus purpureus.

掲載誌: Eur Food Res Technol 2015; 240 (3): 577-582

この研究は、ベニコウジカビ(Monascus purpureus)の二次代謝産物(食用色素の生産に用いられる)の産出に対する、低周波磁界の最適な強度およびばく露時間を調べた。その結果、インキュベーション期間の8-9日目に0.4mT磁界で処理したところ、対照群と比較して、黄色および赤色色素の産出量のピークが、それぞれ65.4%および59.2%増加した。黄色及び赤色色素の産出の動態から、黄色及び赤色色素の産出量は、8-11日目に磁界ばく露したサンプルで対照群よりも有意に増加した。低周波磁界は、1日当たりの産出率を黄色色素で221.25から381.75U/g、赤色色素で165.00から292.50U/gに増加させた。また、黄色色素の生成期間を有意に延長することができた。著者らは、適切な磁界処理によって黄色および赤色色素の産出の効率を高めることができる、と結論付けている。

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研究目的(著者による)

The optimum parameters of exposure to a 50 Hz magnetic field for the production of yellow and red pigment by Monascus purpureus should be investigated in view of a biotechnological use.

詳細情報

Monascus is a traditional fermentation fungus and is used to produce secondary metabolites, which include, among others, the food pigments ankaflavin and monascine.
The optimum parameters for exposure duration, magnetic flux density and exposure period should be determined. To determine the optimum exposure duration, cells were divided into the following groups: exposure to a 0.4 mT magnetic field for 1) 1 h, 2) 4 h, 3) 12 h, 4) 24 h, 5) 48 h, 6) 72 h and 7) 96 h. To determine the optimum magnetic flux density, cells were divided into these groups: exposure for 48 h to a magnetic flux density of 8) 0.05 mT, 9) 0.1 mT, 10) 0.25 mT, 11) 0.4 mT, 12) 0.6 mT, 13) 0.8 mT, 14) 1 mT. To determine the optimum exposure period, cells were divided into the following groups: exposure to a 0.4 mT magnetic field for 48 hours during the 15) 2nd-3rd day, 16) 4-5th day, 17) 5-6th day, 18) 6-7th day, 19) 8-9th day and 20) 10-11th day of cultivation. An additional control group was used for each test.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 48 hours
ばく露2: 50 Hz
ばく露時間: continuous for 48 hours
ばく露3: 50 Hz
ばく露時間: continuous for 48 hours
ばく露4: 50 Hz
ばく露時間: continuous for 1, 4, 12, 24, 48, 72 and 96 hours
ばく露5: 50 Hz
ばく露時間: continuous for 48 hours
ばく露6: 50 Hz
ばく露時間: continuous for 48 hours
ばく露7: 50 Hz
ばく露時間: continuous for 48 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 plastic sample bottles
ばく露装置の詳細 system consisted of five pairs of cylindrical coils (number of threads: 4200, 3600, 3000, 2400, 1800, respectively; outer diameter: 144, 138, 132, 126, 120 mm, respectively; inner diameter: 100 mm; length 600 mm; diameter of wire: 2 mm) wrapped up using magnetic shielding materials; 10 bottles with cells in culture medium were placed on a nonconductive stand in the center of the coils; sample temperature was maintained at 29 ± 1°C by a thermostat
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.05 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.25 mT - 測定値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1, 4, 12, 24, 48, 72 and 96 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.4 mT - 測定値 - -

ばく露5

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.6 mT - 測定値 - -

ばく露6

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.8 mT - 測定値 - -

ばく露7

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Regarding the optimum exposure duration, the highest significant increase in yellow and red pigment production was found in group 5 (48 hours) compared to the control group.
Regarding the optimum magnetic flux density, the highest significant increase in yellow and red pigment production was found in group 11 (0.4 mT) compared to the control group.
Regarding the optimum exposure period, the highest significant increase in yellow and red pigment production was found in group 19 (8th-9th day) compared to the control group.
In a final kinetics study with group 19 over 12 days of incubation time, it was found that the production of yellow and red pigments was significantly increased from the 8th to 11th day in exposed samples compared to the control group, which resulted in 65.4% and 59.2% increase in peak yield of yellow and red pigments, respectively.
The authors conclude, that exposure of Monascus purpureus to a 0.4 mT 50 Hz magnetic field for 48 hours during the 8th and 9th day of exposure may enhance production of yellow and red pigment in fermentation processes.

研究の種別:

研究助成

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