研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト肥満細胞株HMC-1におけるプロテインキナーゼCおよび遺伝子発現に対するマイクロ波ばく露後の影響] med./bio.

Effects on protein kinase C and gene expression in a human mast cell line, HMC-1, following microwave exposure.

掲載誌: Cell Biol Int 2000; 23 (11): 739-748

この研究は、非熱的レベルのマイクロ波ばく露が、ヒト肥満細胞株HMC-1に及ぼす生物学的影響を調べた。細胞へのマイクロ波ばく露には空洞共振器を用い、864.3MHzの連続波を、平均SAR値 7 W / kg、ばく露時間20分間で、3回/日、7日間与えた。ばく露中の細胞培養液の温度は26.5℃に収まっていた。ばく露の影響として、プロテインキナーゼCの局在化への影響;スクリーニングされた588個の遺伝子のうちの3つの遺伝子発現の変化;影響を受けた遺伝子は、がん原遺伝子c-kit、転写因子ヌクレオシドリンキナーゼBおよびアポトーシス関連遺伝子DAD-1であった;ストレス反応遺伝子では、不安定的な増強が見られた;形態学またはF-アクチン分布への有意な影響は検出されなかった、と報告している。

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研究目的(著者による)

To determine the effect of an electromagnetic field exposure in the microwave range (864.3MHz) on protein kinase C and gene expression in human mast cells.

詳細情報

The cells were allowed to cool during the exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 864.3 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 3 x 20 min/day at 4-h intervals, for 7 days
  • SAR: 7.3 W/kg average over mass (cf. remarks) (averaged over 20 g of medium)
  • SAR: 12.1 W/kg unspecified (unspecified) (4.9, 11.4, and 12.1 W/kg at three points)

ばく露1

主たる特性
周波数 864.3 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 3 x 20 min/day at 4-h intervals, for 7 days
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure chamber [Donnellan et al., 1997] consisted of a cubic aluminium box of 1.1 m internal side length and contained a horizontal loop antenna mounted on a horizontal Masonite shelf located at the mid point of the chamber. The reflective walls formed a resonant chamber, and thus a complex modal field pattern was generated that was taken into account in the experimental design.
ばく露装置の詳細 Cells in 20 ml of culture medium in a 75-cm² flask were placed in the exposure chamber.
Sham exposure A sham exposure was conducted.
Additional information The cells were allowed to cool during the exposure. After 20 min, there was less than 1°C difference between the temperature of the culture medium in the exposed (26.5°C) and sham (25.8°C) flasks, and this difference occurred only during the last 5 min of the exposure.
パラメータ
測定量 種別 Method Mass 備考
SAR 7.3 W/kg average over mass 電力損失による決定値 cf. remarks averaged over 20 g of medium
SAR 12.1 W/kg unspecified 測定値 unspecified 4.9, 11.4, and 12.1 W/kg at three points

Reference articles

  • Donnellan M et al. (1997): [835 MHzの電磁放射ばく露による肥満細胞株、RBL-2H3の細胞増殖、形態、分泌能に及ぼす影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

There was no significant morphological difference between the control HMC-1 cells and the exposed cells. There was a consistent trend for an increase in the amount of immunoreactive protein kinase C in the membrane fraction of the exposed cells and a concomitant decrease in the cytosolic fraction. Reproducible and significant changes in expression were seen in only three out of a total of 588 genes screened. The results provide evidence that low-level microwave exposure can affect both gene expression and molecules involved in important cell regulatory functions.

研究の種別:

研究助成

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