この研究は、非熱的レベルのマイクロ波ばく露が、ヒト肥満細胞株HMC-1に及ぼす生物学的影響を調べた。細胞へのマイクロ波ばく露には空洞共振器を用い、864.3MHzの連続波を、平均SAR値 7 W / kg、ばく露時間20分間で、3回／日、7日間与えた。ばく露中の細胞培養液の温度は26.5℃に収まっていた。ばく露の影響として、プロテインキナーゼCの局在化への影響；スクリーニングされた588個の遺伝子のうちの3つの遺伝子の発現の変化；影響を受けた遺伝子は、がん原遺伝子c-kit、転写因子ヌクレオシド二リン酸キナーゼBおよびアポトーシス関連遺伝子DAD-1であった；ストレス反応遺伝子では、不安定的な増強が見られた；形態学またはF-アクチン分布への有意な影響は検出されなかった、と報告している。
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To determine the effect of an electromagnetic field exposure in the microwave range (864.3MHz) on protein kinase C and gene expression in human mast cells.
The cells were allowed to cool during the exposure.
|ばく露時間||repeated daily exposure, 3 x 20 min/day at 4-h intervals, for 7 days|
|チャンバの詳細||The exposure chamber [Donnellan et al., 1997] consisted of a cubic aluminium box of 1.1 m internal side length and contained a horizontal loop antenna mounted on a horizontal Masonite shelf located at the mid point of the chamber. The reflective walls formed a resonant chamber, and thus a complex modal field pattern was generated that was taken into account in the experimental design.|
|ばく露装置の詳細||Cells in 20 ml of culture medium in a 75-cm² flask were placed in the exposure chamber.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||The cells were allowed to cool during the exposure. After 20 min, there was less than 1°C difference between the temperature of the culture medium in the exposed (26.5°C) and sham (25.8°C) flasks, and this difference occurred only during the last 5 min of the exposure.|
There was no significant morphological difference between the control HMC-1 cells and the exposed cells. There was a consistent trend for an increase in the amount of immunoreactive protein kinase C in the membrane fraction of the exposed cells and a concomitant decrease in the cytosolic fraction. Reproducible and significant changes in expression were seen in only three out of a total of 588 genes screened. The results provide evidence that low-level microwave exposure can affect both gene expression and molecules involved in important cell regulatory functions.