研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波にばく露中のげっ歯類赤白血球細胞の分化] med./bio.

Differentiation of murine erythroleukemic cells during exposure to microwave radiation

著者: Brown RF, Marshall SV
掲載誌: Radiat Res 1986; 108 (1): 12-22
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この研究は、ヘキサメチレンビスアセトアミド（HMBA）誘導による赤血球の分化が進行中のマウス赤白血球細胞培養物に、温度可変空気流によって37.4℃に維持しながら、1180MHzマイクロ波（MW）放射の48時間ばく露を与えた。 1180MHzばく露は、電力密度5.5、11、および22mW / cm2で行われ、電力密度当たりのSARは、3.32W / kg / mW / cm2であった。HMBA誘発対照細胞は37.4℃の水槽中で培養された。その結果、ばく露群および対照群の両方において、平均の細胞倍加時間は16.5時間であった；同じく両群の細胞の約65％がベンジジン陽性の分化細胞であった；同じく両群の培養物は、総細胞質タンパク質（mg）当たり約58μgのヘモグロビンを含有した；これらのパラメータに両群で差がないことは、1180MHzおよび類似の周波数でのMW放射は、高温状態がなければ、哺乳類細胞の増殖および分化に影響しないことを示した、と報告している。

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研究目的(acc. to editor)

To study the effect of 1180 MHz microwave radiation on proliferation and differentiation of mammalian cells (murine erythroleukemic cells, MEL) in the absence of hyperthermia.

詳細情報

MEL cells form hemoglobin and show other expressions of erythroid differentiation in response to inducers such as hexamethylene bisacetoneamide, HMBA.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,180 MHz
Modulation type: CW
ばく露時間: continuous for 48 h

ばく露1

主たる特性
周波数 1,180 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 48 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 42.5 cm
チャンバの詳細 A small anechoic chamber made of 1.22 x 1.22-m plywood sheets and lined with MW absorbent pyramids was circulated via a closed-loop Styrofoam duct by warmed air maintained at 37.4 ± 0.2°C.
ばく露装置の詳細 Two sealed thin-walled cellulose nitrate culture tubes (1.6 x 10 cm) containing 12 ml of culture medium were positioned horizontally 6.7 cm apart in the Styrofoam air duct parallel to the E field and equidistant from the antenna by means of a holder (10.2 x 12.6 cm) made of 1-mm balsa sheets with a series of criss-crossed air diffuser vanes.
Additional information Each experiment included two MW irradiated cultures and two non-irradiated controls incubated in a 37.4°C water bath. Irradiated cultures were rotated after 24 h.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 5.5 mW/cm² - 計算値 - -
SAR 18.5 W/kg mean 測定値 - -
電力密度 11 mW/cm² - 計算値 - -
SAR 36.3 W/kg mean 測定値 - -
電力密度 22 mW/cm² - 計算値 - -
SAR 69.2 W/kg mean 測定値 - -
Additional parameter details

The average of power density values measured was about 6% higher than those calculated.

測定および計算の詳細

Power density was calculated from geometry and forward and reflected powers measured by power meters via a dual directional coupler. A broadband radiation meter was used to measure the uniformity of the field. SAR was determined by two methods, one using temperature probes and 60-s decay curves and the other involving Dewar calorimetry [Durney et al., 1980].

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Mean cell doubling time was the same in both the irradiated cultures and the control cultures (16.5 h). About 65% of the cells of exposed cultures and control cultures were benzidine-positive differentiated cells. Both the exposed cultures and control cultures contained approximately 58 µg of hemoglobin/mg total cytoplasmic protein. The absence of any change in these parameters suggests that microwave exposition at 1180 MHz and similar frequencies exerts no effect on proliferation and differentiation of mammalian cells in the absence of hyperthermia.

研究の種別:

研究助成

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