The waveguide applicators consisted of an aluminium tube, 110 mm in diameter and 210 mm long, which was shorted at one end and open at the other. They were fed from two identical excitation probes (monopoles, 33 mm long x 4 mm in diameter) spaced 90° apart and located 50 mm from the shorted end. All six waveguide applicators were housed in a wooden enclosure lined with MW absorbent foam. They were spaced 250 mm apart (center to center) and were separated by a shield consisting of a 200 mm x 300 mm aluminium sheet sandwiched between 6.4-mm thick absorber panels and extending 100 mm higher than the top of the sample dishes.
The sample holder located on the top of the open aperture of the waveguide consisted of two unequal-sized Petri dishes. The inner dish of 60 mm diameter containing the blood cell culture sat concentrically within the outer dish of 150 mm diameter on 3.2-mm plastic standoffs and was circulated by temperature-controlled coolant water maintaining the temperature in the culture at 37 ± 0.5°C. The coolant (130 ml) was kept at the same level as the sample (10 ml) for optimal SAR uniformity.
A total of five separate experiments were conducted over 3 months, each consisting of five RF-field exposed cultures and concurrent sham exposed, negative (incubator) and positive (1.5 Gy137Cs γ radiation; at 0.987 Gy/min) controls.
The SAR for 95% of the sample volume was found to be ±24% of the mean SAR.
Mess- und Berechnungsdetails
The input powers were monitored using a directional coupler and a power meter. Thermometric measurements using a thermistor probe were performed to establish absolute SAR values at various points in the sample, whereas relative E-field measurements were made using an isotropic E-field probe to asses the SAR distribution in the aperture plane.
Franzellitti S et al.
Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay.
Scarfi MR et al.
Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study.
McNamee JP et al.
No evidence for genotoxic effects from 24 h exposure of human leukocytes to 1.9 GHz radiofrequency fields.
McNamee JP et al.
DNA damage in human leukocytes after acute in vitro exposure to a 1.9 GHz pulse-modulated radiofrequency field.
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