Study type: Medical/biological study (experimental study)

Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro. med./bio.

Published in: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2005; 578 (1-2): 149-157

Aim of study (acc. to author)

To study the synergistic DNA damage effects in human lymphocytes induced by 1.8 GHz radiofrequency field irradiation with four chemical mutagens (mitomycin C (DNA crosslinker), bleomycin (radiomimetic agent), methyl methanesulfonate (alkylating agent), and 4-nitroquinoline-1-oxide (UV-mimetic agent)).

Background/further details

Cells were divided into four groups: 1) sham-control group, 2) radiofrequency field irradiation group, 3) chemical exposure group, 4) radiofrequency field irradiation + chemical exposure group. Three combinative exposure ways were: 1) radiofrequency field irradiation before chemical exposure, 2) radiofrequency field irradiation together with chemical exposure, and 3) radiofrequency field irradiation after chemical exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.8 GHz
Exposure duration: continuous for 2 h
  • SAR: 3 W/kg unspecified

Exposure 1

Main characteristics
Frequency 1.8 GHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 2 h
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Chamber The exposure setup was based on two R18 rectangular waveguide chambers, one for RF and one for sham exposure that were blindly activated by a computer controlled signal unit.
Setup Cells were put in 35-mm dishes and placed into the two waveguide chambers (TEM cells).
Sham exposure A sham exposure was conducted.
Additional info The lymphocytes were divided into four groups: sham control, RF exposure, chemical exposure, and RF + chemical exposure group.
Parameters
Measurand Value Type Method Mass Remarks
SAR 3 W/kg unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed no difference of DNA damage indices between radiofrequency field exposed group and control group at 0 and 21 h incubation after exposure.
There were significant differences of DNA damage indices between "mitomycin C group" and "radiofrequency field irradiation + mitomycin C co-exposure group" at 0 and 21 h incubation after treatment.
Also the significant difference of DNA damage indices between "4-nitroquinoline-1-oxide group" and "radiofrequency field irradiation + 4-nitroquinoline-1-oxide co-exposure group" at 0 and 21 h incubation after treatment was revealed.
The DNA damage in "radiofrequency field irradiation + bleomycin co-exposure groups" and "radiofrequency field irradiation + methyl methanesulfonate co-exposure groups" was not significantly increased, as compared with corresponding "bleomycin" and "methyl methanesulfonate groups".
The findings indicated that 1.8 GHz radiofrequency field irradiation for 2 h did not induce the human lymphocyte DNA damage effects in vitro, but it could enhance the human lymphocyte DNA damage effects induced by mitomycin C and 4-nitroquinoline-1-oxide. The synergistic DNA damage effects of 1.8 GHz radiofrequency field irradiation with bleomycin or methyl methanesulfonate were not obvious.

Study character:

Study funded by

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