研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトリンパ球に4種の化学的突然変異原と共に1.8GHz 無線周波電磁界放射が誘発した相乗的損傷作用に関するコメットアッセイによるインビトロ研究] med./bio.

Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro.

掲載誌: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2005; 578 (1-2): 149-157

この研究は、1.8GHz無線周波放射(RFR)と突然変異原である4種の化学物質の併用でヒトのリンパ球誘発されるDNA損傷相乗効果の有無を調べた。一人の健常男性から採取した血液から分離培養したリンパ球で、(1) 擬似ばく露群、(2) RFRばく露群、(3) 化学物質ばく露群、(4) RFR + 各化学物質(ばく露順は3通り)を用意した。組合せばく露の順序は、(1) RFR、各化学物質の順、 (2) 同時ばく露、(3) 各化学物質、RFRの順、である。4種の化学物質は、ミトマイシンC(MMC)、ブレオマイシン(BLM)、メチルメタンスルホン酸塩(MMS)、ニトロチン(4NQO)である。各物質とも4通りの投与濃度で、それぞれ3時間培養した。RFばく露には、2つのR18方形導波管チャンバを用意し、1.8 GHzのGSM信号へのばく露または擬似ばく露をブラインド条件で2時間行った。SARは3 W/kg、温度は37±0.08113 ?Cであった。RFR(2時間ばく露)および/または化学物質(3時間ばく露)の全ての処置終了後、0時間培養および21時間培養したものをコメットアッセイし、テール長(TL)とテールモーメント(TM)を指標にDAN損傷を検査した。その結果、0および21時間培養のどちらでも、RFR群と対照群でDNA損傷に有意差はなかった;同様にどちらの培養でも、MMC群とRFR+MMC組合せばく露群の間、ならびに4NQOg群とRFR+4NQO組合せばく露群の間には、DNA損傷の有意差が見られた(組合せばく露の順序のいずれにおいても);BLMおよびMMSでは、RFRばく露による損傷増加は見られなかった、と報告している。

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研究目的(著者による)

To study the synergistic DNA damage effects in human lymphocytes induced by 1.8 GHz radiofrequency field irradiation with four chemical mutagens (mitomycin C (DNA crosslinker), bleomycin (radiomimetic agent), methyl methanesulfonate (alkylating agent), and 4-nitroquinoline-1-oxide (UV-mimetic agent)).

詳細情報

Cells were divided into four groups: 1) sham-control group, 2) radiofrequency field irradiation group, 3) chemical exposure group, 4) radiofrequency field irradiation + chemical exposure group. Three combinative exposure ways were: 1) radiofrequency field irradiation before chemical exposure, 2) radiofrequency field irradiation together with chemical exposure, and 3) radiofrequency field irradiation after chemical exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
ばく露時間: continuous for 2 h
  • SAR: 3 W/kg unspecified

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2 h
Modulation
Modulation type unspecified
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure setup was based on two R18 rectangular waveguide chambers, one for RF and one for sham exposure that were blindly activated by a computer controlled signal unit.
ばく露装置の詳細 Cells were put in 35-mm dishes and placed into the two waveguide chambers (TEM cells).
Sham exposure A sham exposure was conducted.
Additional information The lymphocytes were divided into four groups: sham control, RF exposure, chemical exposure, and RF + chemical exposure group.
パラメータ
測定量 種別 Method Mass 備考
SAR 3 W/kg unspecified 指定なし - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed no difference of DNA damage indices between radiofrequency field exposed group and control group at 0 and 21 h incubation after exposure.
There were significant differences of DNA damage indices between "mitomycin C group" and "radiofrequency field irradiation + mitomycin C co-exposure group" at 0 and 21 h incubation after treatment.
Also the significant difference of DNA damage indices between "4-nitroquinoline-1-oxide group" and "radiofrequency field irradiation + 4-nitroquinoline-1-oxide co-exposure group" at 0 and 21 h incubation after treatment was revealed.
The DNA damage in "radiofrequency field irradiation + bleomycin co-exposure groups" and "radiofrequency field irradiation + methyl methanesulfonate co-exposure groups" was not significantly increased, as compared with corresponding "bleomycin" and "methyl methanesulfonate groups".
The findings indicated that 1.8 GHz radiofrequency field irradiation for 2 h did not induce the human lymphocyte DNA damage effects in vitro, but it could enhance the human lymphocyte DNA damage effects induced by mitomycin C and 4-nitroquinoline-1-oxide. The synergistic DNA damage effects of 1.8 GHz radiofrequency field irradiation with bleomycin or methyl methanesulfonate were not obvious.

研究の種別:

研究助成

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