Study type: Medical/biological study (experimental study)

Impact of 1.8-GHz radiofrequency radiation (RFR) on DNA damage and repair induced by doxorubicin in human B-cell lymphoblastoid cells. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2010; 695 (1-2): 16-21

Aim of study (acc. to author)

To study whether 1.8 GHz radiofrequency irradiation (2 W/kg) can influence DNA repair in human B cell lymphoblastoid cells exposed to doxorubicin (at the doses of 0 µg/ml, 0.05 µg/ml, 0.075 µg/ml, 0.10 µg/ml, 0.15 µg/ml and 0.20 µg/ml).

Background/further details

Five types of combinative exposures of radiofrequency plus doxorubicin were tested: 1) radiofrequency exposure (2 h) before, during and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 2) radiofrequency exposure before and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 3) radiofrequency exposure during and for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 4) radiofrequency exposure for 6 h, 12 h, 18 h and 24 h after doxorubicin exposure, 5) sham exposure before, during and after doxorubicin exposure.
DNA damage was detected at 0 h, 6 h, 12 h, 18 h and 24 h after exposure to doxorubicin.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.8 GHz
Modulation type: pulsed
Exposure duration: continuous for up to 28 h

General information

Cells were treated in five groups: i) EMF exposure before (2 h), during (2h) and after (6 h, 12 h, 18 hr, 24 h) exposure to DOX ii) EMF exposure before (2 h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX iii) EMF exposure during (2h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX iv) EMF exposure after (6 h, 12 h, 18 h, 24 h) exposure to DOX vi) Sham exposure before (2 h), during (2 h) and after (6 h, 12 h, 18 h, 24 h) exposure to DOX

Exposure 1

Main characteristics
Frequency 1.8 GHz
Type
Exposure duration continuous for up to 28 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Exposure setup
Exposure source
Setup probes put inside the waveguide which was placed inside an incubator; dishes positioned in the H-field maximum of the standing wave
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg - - - -

Reference articles

  • Zeng Q et al. (2006): Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF-7 cells.
  • Leszczynski D et al. (2004): Applicability of discovery science approach to determine biological effects of mobile phone radiation.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that 1) radiofrequency exposure could not directly induce DNA damage of human B cell lymphoblastoid cells; 2) doxorubicin could significantly induce DNA damage of the lymphoblastoid cells with the dose-response relationship, and there were special DNA repair characteristics of DNA damage induced by doxorubicin; 3) combinative exposure (type 1: exposure to radiofrequency for 2 h, then simultaneous exposure to radiofrequency and doxorubicin, and exposure to radiofrequency for 6 h, 12 h, 18 h and 24 h after exposure to doxorubicin) could obviously influence DNA repair at six and twelve hours after exposure to doxorubicin for four doxorubicin doses (0.075 µg/ml, 0.10 µg/ml, 0.15 µg/ml and 0.20 µg/ml).
The authors conclude, that the data indicate that 1.8 GHz radiofrequency exposure (2 W/kg) did not induce DNA damage directly but may influence the DNA repair of the DNA damage induced by doxorubicin to some extent.

Study character:

Study funded by

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