Study type: Medical/biological study (experimental study)

Evaluation of genotoxic effects in human peripheral blood leukocytes following an acute in vitro exposure to 900 MHz radiofrequency fields. med./bio.

Published in: Bioelectromagnetics 2005; 26 (4): 258-265

Aim of study (acc. to author)

To investigate the induction of genotoxic effects on human peripheral blood leukocytes following 2 h exposure to 900 MHz (GSM signal) radiofrequency irradiation.

Background/further details

Positive controls were also provided by using methyl methanesulfonate (for comet assay) and mitomycin C (for chromosome aberration or sister chromatid exchange tests).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 2 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Pulse type rectangular
Additional info

The signal was both phase modulated by Gaussian minimum shift keying (GMSK) and amplitude modulated (rectangular pulses, 217 Hz) according to the TDMA technique. The modulating bit rate was 1/T = 270.833 kbit/s and the 3 dB bandwidth, B, was such that BT = 0.3. Voice modulation was simulated by sending a 9 bit pseudorandom sequence.

Exposure setup
Exposure source
Chamber The exposure chamber was made of a rectangular waveguide (124 x 248 x 500 mm), surrounded by a thermostated (37.0 ± 0.1 °C) water jacket, and two coax-to-waveguide adapters. The 8-ml samples were located in four T25 culture flasks inserted into the waveguide. The narrow side of the waveguide and the E field were horizontal, thus parallel to the culture liquid layer, which was 3 mm thick.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.3 W/kg mean measured and calculated unspecified -
SAR 1 W/kg mean measured and calculated unspecified -

Reference articles

  • Kuster N et al. (2000): Recommended minimal requirements and development guidelines for exposure setups of bio-experiments addressing the health risk concern of wireless communications.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No statistically significant differences were found in exposed samples in comparison with sham-exposed ones for all the parameters investigated. On the contrary, the positive controls gave a statistically significant increase in DNA damage in all cases.
The data do not support the hypothesis that 900 MHz radiofrequency field exposure induces DNA damage in human peripheral blood leukocytes.

Study character:

Study funded by

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