Study type: Medical/biological study (experimental study)

Effects of 50 Hz electromagnetic fields on the histology, apoptosis, and expression of c-Fos and beta-catenin on the livers of preincubated white Leghorn chicken embryos. med./bio.

Published in: Electromagn Biol Med 2011; 30 (3): 158-169

Aim of study (acc. to author)

To study the effects of sinusoidal electromagnetic fields on histopathology, apoptosis, and gene expressions (c-fos and beta-catenin) in the livers of chicken embryos.

Background/further details

300 fertilized eggs were divided into four groups of exposure (n=40, each, incubated for 13, 14, 15 and 19 days), a control group (n=70), and a sham exposure group (n=70). 13-15 and 19-day old chicken embryos were removed from the shells (following incubation) and the livers were investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 24 h before incubation

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 24 h before incubation
Exposure setup
Exposure source
Setup 42 cm long cylindrical coil with an inner diameter of 9.6 cm, an outer diameter of 11.5 cm and 980 turns of 2.5 mm diameter enameled copper wire; magnetic flux parallel to the long axis of the embryo's body; coil chamber ventilated
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7.32 mT - - - -

Reference articles

  • Shams Lahijani M et al. (2009): Histopathological and ultrastructural studies on the effects of electromagnetic fields on the liver of preincubated white leghorn chicken embryo.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed extensive hemorrhages all over the chicken embryos' bodies and livers, more lymphoid tissues, disturbed parenchymal tissues, sinusoid denaturation, an increase in the number of apoptotic cells, and a decrease in gene expressions of c-fos and beta-catenin in the livers of all exposure groups compared with the control group and sham exposure group.
The authors conclude that the magnetic field exposure has drastic effects on the livers of 13, 14, 15, and 19 day-old chicken embryos as presented by histopathological changes.

Study character:

Study funded by

Related articles