研究のタイプ: 医学/生物学の研究 (experimental study)

[マウス海馬細胞株HT22のDNA損傷反応への60Hz磁界の影響] med./bio.

Effects of 60-Hz magnetic fields on DNA damage responses in HT22 mouse hippocampal cell lines.

掲載誌: JEES 2015; 15 (3): 123-128

この研究は、非腫瘍細胞であるマウス海馬細胞株HT22のDNA損傷反応への超低周波磁界(ELF-MF: 60H、2mT)の影響を調べた。その結果、磁界ばく露により、γH2AX発現増加、コメットテイルモーメント、異数体形成は見られなかった、と報告している。

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研究目的(著者による)

The genotoxic effects of exposure of mouse neuronal cells to a 60 Hz magnetic field should be investigated.

詳細情報

In previous studies by the authors, the genotoxic effects of extremely low frequency magnetic fields in several epithelial and fibroblast cell lines had been investigated (Lee et al. 2012, Jin et al. 2014, Jin et al. 2012, Jin et al. 2015, Yoon et al. 2014). With this article, the study series has been expanded by research on neuronal cells.
Mouse hippocampus neuronal cells were divided into two groups: exposure to the magnetic field and sham exposure.
A positive control was performed. All results were obtained from at least three replicate experiments.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 4, 6, 12, 16 or 24 hours

General information

positive controls were exposed to gamma radiation (10 Gy as a single dose at a rate of 5 Gy/min)

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4, 6, 12, 16 or 24 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 exposure chamber in CO2 incubator
ばく露装置の詳細 cells in 100 mm cell culture dishes were placed in the exposure chamber inside the incubator; the exposure chamber was equipped with coils and was shielded; during exposure, the temperature in the chamber was maintained at 37 ± 0.3°C by circulating water and the temperature of the culture medium was monitored at 2-hour intervals; the incubator had a humidified atmosphere of 95% air and 5% CO2
Sham exposure A sham exposure was conducted.
Additional information sham exposed cells were treated in another incubator under the same experimental procedures with the exception of ELF-MF exposure
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

Reference articles

  • Lee HJ et al. (2012): [NIH3T3細胞における細胞の形質転換に対する各種のストレス要因と60Hz電磁界ばく露の複合的な影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After 6 hours of exposure, cell numbers were significantly higher in the exposure group compared to the sham exposure group. All other parameters did not show any significant differences at any time point between exposed and sham exposed cells.
The authors conclude that exposure of mouse neuronal cells to a 60 Hz magnetic field has no genotoxic effects. In synopsis with the previous studies, they see a varying sensitivity of different cell lines to genotoxic effects.

研究の種別:

研究助成

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