Study type: Medical/biological study (experimental study)

Consequences of electromagnetic field (EMF) radiation during early pregnancy - androgen synthesis and release from the myometrium of pigs in vitro. med./bio.

Published in: Anim Reprod Sci 2020; 218: 106465

Aim of study (acc. to author)

The effects of exposure to a 50 Hz or 120 Hz magnetic field on steroidogenic activity of myometrium collected from pigs should be investigated.

Background/further details

Myometrial samples were collected from female pigs after 15-16 of pregnancy and divided into the following groups: 1) exposure to the 50 Hz magnetic field for 2 hours, 2) exposure to the 120 Hz magnetic field for 2 hours, 3) control group for 2 hour-exposure, 4) exposure to the 50 Hz magnetic field for 4 hours, 5) exposure to the 120 Hz magnetic field for 4 hours, 6) control group for 4 hour-exposure. To determine whether progesterone functions as a protectant from magnetic field exposure, some samples were treated with progesterone.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 2 or 4 hours
Exposure 2: 120 Hz
Exposure duration: 2 or 4 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 2 or 4 hours
Exposure setup
Exposure source
  • Magneris system
Distance between exposed object and exposure source 5 cm
Chamber 24-well plates
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 mT - - - -

Exposure 2

Main characteristics
Frequency 120 Hz
Type
Waveform
Exposure duration 2 or 4 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 mT - - - -

Reference articles

  • Koziorowska A et al. (2018): Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17β (E2) in uterine tissues: An in vitro study.
  • Koziorowska A et al. (2016): The impact of electromagnetic field at a frequency of 50 Hz and a magnetic induction of 2.5 mT on viability of pineal cells in vitro.

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Without progesterone treatment, exposure to the 50 Hz magnetic field (group 1) and 120 Hz magnetic field for 2 hours (group 2) significantly decreased androstenedione release compared to the control group. With progesterone treatment, testosterone release was signifantly decreased compared to the control group. Exposure to the 50 Hz magnetic field significantly increased the gene expression of HSD3B1 and level of cytochrome P450c17 after 4 hours of exposure (group 4) without progesterone treatment compared to the control group. Exposure to the 120 Hz magnetic field for 4 hours (group 5) significantly decreased androstenedione release compared to the control group without progesterone treatment. With progesterone treatment, CYP17A1 gene expression was significantly decreased and the level of 3βHSD and androstenedione release were significantly increased compared to the control group.
The authors conclude that exposure to a 50 Hz or 120 Hz magnetic field might alter steroidogenic activity of myometrium collected from pigs without an influence of progesterone.

Study character:

Study funded by

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