Study type: Medical/biological study (experimental study)

Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology. med./bio.

Published in: Biomed Res Int 2021; 2021: 8510315

Aim of study (acc. to author)

The genotoxic effects of exposure of different human cell lines to 50 Hz magnetic fields should be investigated.

Background/further details

Human amnion epithelial cells, human skin fibroblast cells, and human umbilical vein endothelial cells were exposed to magnetic fields with magnetic flux densities of 0.4, 1, and 2 mT for 15 min, 1 hour, and 24 hours, respectively. For each exposure group, a separate sham exposure was conducted. Two dishes were included in each group and the experiments were repeated three times.
Positive controls were treated with 4-nitroquinoline 1-oxide, a chemical known for inducing DNA damage.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 15 minutes, 1 hour and 24 hours
Exposure 2: 50 Hz
Exposure duration: 15 minutes, 1 hour and 24 hours
Exposure 3: 50 Hz
Exposure duration: 15 minutes, 1 hour and 24 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 15 minutes, 1 hour and 24 hours
Exposure setup
Exposure source
Chamber 35-mm diameter Petri dishes in exposure chambers at 37 °C and 5% CO2
Setup sXc-ELF exposure system had an incubator consisting of two identical chambers that contained a series of Helmholtz coils; one chamber was used for exposure, the other was for the sham exposure using an opposite phase connection; an air cooling system kept the temperature differences between the two chambers at less than 0.1 °C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 15 minutes, 1 hour and 24 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 15 minutes, 1 hour and 24 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Reference articles

  • Zhu K et al. (2016): Extremely low frequency magnetic fields do not induce DNA damage in human lens epithelial cells in vitro.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No genotoxic effects of exposure of different human cell lines to 50 Hz magnetic fields were found.

Study character:

Study funded by

Related articles