研究のタイプ: 医学/生物学の研究 (experimental study)

[CHO-K1細胞での小核形成に対する高周波電磁界の影響] med./bio.

Effects of high frequency electromagnetic fields on micronucleus formation in CHO-K1 cells.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2003; 541 (1-2): 81-89

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

To investigate whether exposure to high frequency electromagnetic fields at a wide range of average SARs from 13 to 100 W/kg with input powers of 7.8 and 13 W could lead to increased micronucleus formation.

詳細情報

Micronucleus formation induced by a high frequency electromagnetic field alone, with bleomycin alone (since bleomycin is known to be a clastogen), or a combination of both was examined. To take the increased temperature into account, the frequency of micronucleus induced was compared to that in cells incubated at 39°C.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 18 h
  • 電力: 7.8 W (input power)
  • SAR: 50 W/kg mean (in the middle ring)
  • SAR: 39 W/kg mean (in the outer ring)
  • SAR: 13 W/kg mean (in the inner ring)
ばく露2: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 18 h
  • 電力: 13 W (input power)
  • SAR: 100 W/kg mean (in the middle ring)
  • SAR: 78 W/kg mean (in the outer ring)
  • SAR: 25 W/kg mean (in the inner ring)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 18 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The incubator-like atmosphere inside the cavity with 95% air and 5% CO2 was maintained at 37°C.
ばく露装置の詳細 Cell suspensions of 3.0, 12.8, and 27.4 ml volume were plated, incubated, and exposed in the three radial compartments (inner, middle, and outer ring) of an annular culture plate.
Sham exposure A sham exposure was conducted.
Additional information As a high temperature control, cells were incubated at 39°C for 18 h.
パラメータ
測定量 種別 Method Mass 備考
電力 7.8 W - 測定値 - input power
SAR 50 W/kg mean 推定値 - in the middle ring
SAR 39 W/kg mean 推定値 - in the outer ring
SAR 13 W/kg mean 推定値 - in the inner ring

ばく露2

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 18 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
Additional information Cells were also treated with bleomycin alone (positive control) or followed by RF exposure at an input power of 13 W.
パラメータ
測定量 種別 Method Mass 備考
電力 13 W - 測定値 - input power
SAR 100 W/kg mean 推定値 - in the middle ring
SAR 78 W/kg mean 推定値 - in the outer ring
SAR 25 W/kg mean 推定値 - in the inner ring

Reference articles

  • Tian F et al. (2002): [2.45 GHz電磁界ばく露はヒトグリオーマMO54細胞での20W/kg以上の高SARでhsp70を誘導するが5W/kgでは誘導しない]
  • Guy AW (1987): [非電離放射線へのばく露に関するドシメトリー:極低周波からマイクロ波まで]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The micronucleus frequency of cells exposed to a high frequency electromagnetic field at a SAR of up to 50 W/kg was not different to that in sham-expsoed cells. There were statistically significant increases in the micronucleus frequencies of cells in the middle and outer wells of the annular culture plate caused by exposure to a high frequency electromagnetic field at 100 and 78 W/kg, respectively. Although there was an increase of micronucleus formation at 39°C compared with a sham-exposed culture, the rate was lower than after exposure to high frequency electromagnetic field. Furthermore, radiofrequency electromagnetic exposure enhances the frequency of micronuclei after bleomycin-tretament.

研究の種別:

研究助成

関連論文