研究のタイプ: 医学/生物学の研究 (experimental study)

[1.8 GHz振幅変調無線周波電磁界にばく露したヒト絨毛細胞株におけるHSP70発現とDNA損傷の評価] med./bio.

Evaluation of HSP70 expression and DNA damage in cells of a human trophoblast cell line exposed to 1.8 GHz amplitude-modulated radiofrequency fields.

掲載誌: Radiat Res 2008; 169 (3): 270-279

この研究は、ヒト絨毛細胞株HTR-8/Svneoでのタンパク質発現HSP70,HSC70)、遺伝子発現(HSP70A,B,CおよびHSC70)および一次的DNA損傷反応に対する非熱的レベルの217Hzパルス変調1817MHz正弦波(EMFs:ばく露時間:1時間、SAR:2 W/kg)の影響を調べた。その結果、EMFsばく露群では、HSP70またはHSC70のタンパク質および遺伝子発現有意な変化がなかった;DNA鎖切断への影響は観察されなかった、と報告している。

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研究目的(著者による)

To determine whether radiofrequency electromagnetic fields could induce cellular effects in trophoblast cells.

詳細情報

The effects observed after radiofrequency exposure were compared to those caused by heat (43°C, 1 h) or hydrogen peroxide, which were used as positive controls.
Trophoblast cells have not been used previously in similar experiments. However, their high proliferation and invasion capability, their fine modulation by physiological factors, and their high sensitivity to external stimuli, make these cells an attractive model for investigating possible detrimental effects of radiofrequency electromagnetic fields on cell physiology.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,817 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h
  • SAR: 2 W/kg average over time

ばく露1

主たる特性
周波数 1,817 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
特性
  • guided field
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

TDMA GSM scheme [Pedersen, 1997] with every 26th frame being idle, adding an 8 Hz modulation to the signal

ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure system [Schonborn et al., 2000] located inside an incubator consisted of two 128.5 x 65 x 424 mm³ brass single-mode waveguide resonators (equipped with DC ventilators) that were assigned to exposure or sham condition by the computer-controlled signal unit ensuring blind conditions for the experiment.
ばく露装置の詳細 Each resonator was equipped with a plastic holder holding six 35-mm Petri dishes arranged in two stacks in the H-field maxima of the standing wave (E-polarization).
Sham exposure A sham exposure was conducted.
Additional information More than 90% of the power coupled into the waveguides was reflected and directed into a 50-Ω terminator via the circulator (included in the amplifier).
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg average over time 測定値および計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

No evidence was found that a 1 h exposure to GSM 217 Hz induced a HSP70-mediated stress response or primary DNA damage in HTR-8/SVneo cell line.

研究の種別:

研究助成

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