研究のタイプ: 医学／生物学の研究 (experimental study)

[全身マイクロ波照射と抗酸化薬投与後の腫瘍マウスの細胞中の腫瘍ネクローシス因子の産出] med./bio.

The Production of Tumor Necrosis Factor in Cells of Tumor-Bearing Mice after Total-Body Microwave Irradiation and Antioxidant Diet

著者: Novoselova EG, Ogay VB, Sorokina OV, Glushkova OV, Sinotova OA, Fesenko EE
掲載誌: Electromagn Biol Med 2004; 23 (2): 167-180
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この研究は、健康なマウスおよび担がんマウス（TBM）のマクロファージおよびTリンパ球における腫瘍壊死因子（TNF）産生に対する弱いマイクロ波（MW）（8.15-18 GHz、1 µW/ cm2、1日1.5時間）の反復ばく露および酸化防止剤（AO）（β-カロチン、a-トコフェロール、ユビキノンQ 9）の摂取の影響を調べた。また、TBMの腫瘍サイズおよび死亡率も追跡調査した。その結果、MWばく露およびAO食餌は、健康なマウスの細胞におけるTNF産生を刺激した；初期段階では、腫瘍成長がマウス細胞のTNF産生を引き起こしたが、この効果は腫瘍成長とともに低下した；MWばく露を受けたTBMでは、TNF産生が無ばく露TBMよりも高かった；反対に、AO食餌は、健康なマウスのTNF産生を誘発したが、TBMでのTNF分泌には影響しなかった；したがって、TBMのMW長期処置（ただしAO食餌処置は無しでの）は腫瘍成長速度を低下させ、全体的に見た動物の寿命を延ばした、と報告している。

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研究目的（著者による）

To study the effects of repeated treatment with weak microwaves and diet with antioxidants (beta-carotene, alpha-tocopherol, and ubiquinone Q 9) on production of tumor necrosis factor in macrophages and T lymphocytes of healthy and tumor-bearing mice.

詳細情報

Feeding and microwave exposure were started on day 1 after transplantation of cancer cells. The animal cancer model was produced by subcutaneous injection of 2 x 105 Ehrlich ascites carcinoma cells in one hind limb. The mice were killed by decapitation of days 7, 14, and 30 after transplantation of cancer cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 8.15–18 GHz

ばく露1

主たる特性
周波数 8.15–18 GHz
タイプ
  • electromagnetic field
Additional information The frequency stability was better than 5%, and spectral width was better than 1 MHz. Higher harmonics were less than 25 dB.
Modulation
Modulation type cf. additional information
Additional information

Sweep frequency between 8.15 and 18 GHz with 1 s direct sweep time and 16 ms of reversed time
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 80 cm
ばく露装置の詳細 animals kept in 25 cm x 25 cm x 45 cm plastic cages
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 5 mW/kg maximum 推定値 - 2 mW/kg - 5 mW/kg
電力密度 1.6 µW/cm² maximum 測定値 - at the center of the cage
電力密度 0.2 µW/cm² minimum 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

Microwave exposure and antioxidant diet stimulated production of tumor necrosis factor in cells from healthy animals. At early stages, tumor growth induced tumor necrosis factor production in cells; however, this effect decreased as tumors grew. In tumor-bearing mice exposed to microwaves, tumor necrosis factor production was higher than in unexposed tumor-bearing animals.
Oppositely, antioxidant diet induced tumor necrosis factor production in healthy animals but did not affect tumor necrosis factor secretion in tumor-bearing mice. Accordingly, prolonged treatment of tumor-bearing mice to microwaves, but not to antioxidant diet, decreased tumor growth rate and increased overall animal longevity.
These data suggest that diminished tumor growth rate due to extremely low-level microwave exposure of mice carrying tumors, at least in part, was caused by enhancement in tumor necrosis factor production and accumulation of plasma tumor necrosis factor.

研究の種別:

研究助成

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