研究のタイプ: 医学/生物学の研究 (experimental study)

[スイスアルビノマウスの発がんに対する低レベルのマイクロ波の影響] med./bio.

Effects of low level microwave radiation on carcinogenesis in Swiss Albino mice.

掲載誌: Mol Cell Biochem 2011; 348 (1-2): 191-197

この研究は、発がん性物質と低レベルの無線周波RF: 16Hzで振幅変調した112MHz電磁界SAR:0.75 W/kg)またはマイクロ波MW: 2.45GHz電磁界SAR:0.1 W/kg)の組合せばく露による発がん影響をスイスアルビノ系雄マウスで調べた。研究は2つの部分に分かれ、Part Aは、100μg/固体の7,12-ジメチルベンゾアントラセン(DMBA)をマウスの皮膚に局所的に単回投与し、次に無ばく露RFばく露(TEMセル内)、MWばく露(無響チャンバー内)を1日2時間、週3日で16週間継続した。この他にDMBA投与無しのRFばく露群、MWばく露群などとも比較した。Part Bは、マウス1匹当たり8×108個の腹水がん細胞(エールリッヒ・レトレ腹水E系統株)を腹腔内投与し、2種類の電磁界への擬似ばく露群、ばく露群(1日2時間、14日間)の4群について比較した。その結果、RFまたはMWばく露群では腫瘍発生は見られなかった;DMBAの単回投与後のRF/MWばく露群においても、マウスの皮膚に目で見て分かるような腫瘍は発生しなかった;腹水がん細胞の腹腔内移植後にRF/MWばく露させた群では、擬似ばく露群に比べ、細胞数に有意でない若干の増加が見られた、などを報告している。

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研究目的(著者による)

To study the effect of low level radiofrequency (112 MHz amplitude modulated at 16 Hz) and 2.45 GHz exposures on mice sensitized to tumor initiated by DMBA or transplanted with ascites carcinoma cells.

詳細情報

The study was divided into two parts. Part A: a single dose of the chemical carcinogen DMBA (100 µg/animal) was applied on the skin of the mice and were exposed to 112 MHz or to 2.45 GHz (2 h/day, 3 days/week for 16 weeks). In part A seven groups (n=18) were included: 1) sham exposure group, 2) DMBA group, 3) DMBA + 112 MHz exposure group, 4) DMBA + 2.45 GHz exposure group, 5) 112 MHz exposure group, 6) 2.45 GHz exposure group, 7) positive control: DMBA + croton oil (i.e. with TPA, as tumor promoter).
Part B: mice were transplanted intraperitoneally with ascites carcinoma cells (Ehrlich-Lettre ascites, strain E; 8 x 108 per mouse). These mice were exposed to 112 MHz or 2.45 GHz for a period of 14 days. The following groups (n=8) were included: two different sham exposure groups, 112 MHz exposure group, and 2.45 GHz exposure group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 112 MHz
Modulation type: AM
ばく露時間: experiment A: continuous for 2 h/day, 3 days/week for 16 weeks; Experiment B: continuous for 2 h/day for 14 days
ばく露2: 2.45 GHz
ばく露時間: experiment A: continuous for 2 h/day, 3 days/week for 16 weeks; experiment B: continuous for 2 h/day on 14 days

General information

animals used in the experiments A and B were divided into several groups: experiment A: i) sham exposure ii) application of a single dose of 7,12-dimethylbenz(a)anthracene (DMBA) iii) application of a single dose of DMBA + exposure to 112 MHz electromagnetic field iv) application of a single dose of DMBA + exposure to 2.45 GHz electromagnetic field v) exposure to 112 MHz electromagnetic field vi) exposure to 2.45 GHz electromagnetic field vii) application of a single dose of DMBA + tumor promoter experiment B: i) sham exposure to 112 MHz ii) exposure to 112 MHz iii) sham exposure to 2.45 GHz iv) exposure to 2.45 GHz

ばく露1

主たる特性
周波数 112 MHz
タイプ
  • electromagnetic field
ばく露時間 experiment A: continuous for 2 h/day, 3 days/week for 16 weeks; Experiment B: continuous for 2 h/day for 14 days
Modulation
Modulation type AM
Modulation frequency 16 Hz
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two mice placed in a 32 cm x 10 cm x 9 cm Plexiglas cage, positioned in the center of the rectangular TEM cell
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 1 mW/cm² - 計算値 - -
SAR 0.75 W/kg - - - -

ばく露2

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 experiment A: continuous for 2 h/day, 3 days/week for 16 weeks; experiment B: continuous for 2 h/day on 14 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 anechoic chamber made of wood; 9 mice placed in pre-specified compartments of a 43 cm x 27 cm x 15 cm Plexiglas cage; cage placed in the anechoic chamber
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.34 mW/cm² - 計算値 - -
SAR 0.1 W/kg - - - for a small mouse

Reference articles

  • Paulraj R et al. (1999): [振幅変調RFに慢性的ばく露されたラット脳のカルシウムイオン流出とODC活性に関する影響]
  • Lin JC et al. (1979): [マウスの成長および血液学的指標に対する148MHz無線周波の反復ばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The data did not show any statistically significant differences in the overall skin tumor incidence, latency to skin tumor onset, number of tumors per tumor bearing mice, or tumor size between the 112 MHz or 2.45 GHz exposure and sham exposure groups. Similarly, an application of a single dose of DMBA followed by 112 MHz or 2.45 GHz exposure also did not produce any visible extra tumors on the skin of mice.
On the other hand, mice transplanted with ascites carcinoma cells showed a slight increase in the cell numbers as compared to the control group. However, the increase was insignificant.
These data show that low level radiofrequency or microwave exposure do not alter tumor growth and development.

研究の種別:

研究助成

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