研究のタイプ: 医学/生物学の研究 (experimental study)

[前がん性乳房上皮細胞の増殖に対する超広帯域電磁パルスの影響] med./bio.

Effects of ultra-wideband electromagnetic pulses on pre-neoplastic mammary epithelial cell proliferation.

掲載誌: Cell Prolif 2005; 38 (3): 153-163

この研究は、前腫瘍性CL-S1乳腺上皮細胞のインビトロでの増殖に対するナノパルス電磁界(強度は低から中程度)の長期ばく露の影響を調べた。細胞はコミトゲンとして10 ng/ ml のEGFおよび10 μg/ mlのインスリンを含む無血清規定培地増殖・維持された。ナノパルス電磁界電界強度は18 kV/ m、パルス繰り返し周波数1 kHzパルス幅10 nsであり、ばく露時間は0.25 - 3.0時間の短時間ばく露および4 - 6 時間の長時間ばく露を行なった。その後72時間培養した。その結果、ナノパルスの短時間ばく露の場合、その後の72時間培養中のCL-S1細胞の増殖または生存率に影響は見られなかった;ナノパルスの長時間ばく露の場合、無処理対照と比較して、細胞増殖が有意に増加した;追加の実験として、EGFのみを含む培地細胞を維持した場合、ナノパルスばく露によりCL-S1細胞の増殖促進されたが、マイトジェンを含まない、またはインスリンのみを含む規定培地で維持された細胞では影響がなかったことが示された、などの知見を報告している。

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研究目的(著者による)

To study the effects of prolonged exposure to non-ionizing, low to moderate intensity nanopulses on the growth of pre-neoplastic CL-S1 mammary epithelial cells in vitro. Additional studies investigated the effects of nanopulse exposure on the activation of the mitogen-activated protein kinase (MAPK) mitogenic signalling pathway in these cells.

詳細情報

Electromagnetic ultra-wideband pulses or nanopulses, are generated by a wide range of electronic devices used in communications, radar technology, and high-powered microwave weapons.
Cells were grown in culture medium containing 10 ng/ml EGF (epidermal growth factor) and 10 µg/ml insulin as co-mitogens.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
Modulation type: pulsed
ばく露時間: continuous for 1/4, 1, 2 and 4 hours
ばく露2:
Modulation type: pulsed
ばく露時間: continuous for 4 hours
ばく露3:
Modulation type: pulsed
ばく露時間: continuous for 1, 2, 3, 4, 5 and 6 hours

General information

All experiments were repeated at least three times.

ばく露1

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
特性
  • guided field
ばく露時間 continuous for 1/4, 1, 2 and 4 hours
Modulation
Modulation type pulsed
Pulse width 10 ns
Rise time 0.1 ns
Repetition frequency 1,000 kHz
Additional information

Pulse repetition rates tested were 1, 10, 100 and 1000 kHz.

ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure setup was installed in a copper plate shielded room with an attenuation of 85 dB at 10 GHz. Ancillary equipment was located in a second copper mesh shielded room.
ばく露装置の詳細 Biological samples were placed in the temperature-controlled (27 °C) gigahertz transverse electromagnetic mode (GTEM) cell and exposed to nanopulses of defined properties. These pulses are non-ionizing and do not cause sample heating.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 18 kV/m - - - -

ばく露2

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
特性
  • guided field
ばく露時間 continuous for 4 hours
Modulation
Modulation type pulsed
Pulse width 10 ns
Rise time 0.1 ns
Repetition frequency 1,000 kHz
Additional information

Pulse repetition rates tested were 1, 10, 100 and 1000 kHz.

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 0.18 kV/m - - - -
電界強度 1.8 kV/m - - - -

ばく露3

主たる特性
周波数
タイプ
  • electromagnetic field
波形
  • pulsed
特性
  • guided field
ばく露時間 continuous for 1, 2, 3, 4, 5 and 6 hours
Modulation
Modulation type pulsed
Pulse width 10 ns
Rise time 0.1 ns
Repetition frequency 1 kHz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 18 kV/m - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that 0.25-3.0 h exposure to nanopulses (18 kV/m field intensity, 1 kHz repetition rate, 10 ns pulse width) had no effect on cell growth or cell viability during the subsequent 72-h culture period. However, exposure to similar nanopulses for prolonged periods of time (4-6 h) resulted in a significant increase in cell proliferation, as compared to untreated controls.
Nanopulse exposure enhanced cell growth when cells were maintained in media containing only EGF, but had no effect on cells maintained in defined media that were mitogen-free or containing only insulin.
The findings also revealed that the growth-promoting effects of nanopulse exposure were associated with a relatively large increase in intracellular levels of phospho-MEK1 and phospho-ERK1/2 in these cells.
These results demonstrate that prolonged exposure to moderate levels of ultra-wideband pulses enhanced EGF-dependent mitogenesis, and that this growth-promoting effect appears to be mediated by enhanced activation of the mitogen-activated protein kinase signalling pathway in these cells.

研究の種別:

研究助成

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