この研究は、血液脳関門（BBB）のインビトロモデルとして機能するブタ脳の微小血管内皮細胞培養（PBEC）のバリア緊密性、輸送プロセス、および形態に、汎用UMTSのRF電磁界が影響を与えるか否かを調べた。オンライン監視システムを組み込んだばく露装置を開発し、ばく露と経内皮電気抵抗（TEER）測定を同時に行えるようにした。TEERはBBBの緊密性を決定づける。PBECに、平均電界強度3.4〜34 V / mでのばく露を最長84時間連続して与えた。このばく露レベルは、最大1.8 W / kgのSARとなるため、非熱的条件は確保された。結果として、RF電磁界によるBBBの機能障害の証拠は１つも見つからなかった；ばく露後およびばく露中の14C-スクロースおよび血清アルブミンの透過ならびにTEERを用いて、BBB緊密性を定量化したが、擬似ばく露群と比較して違いはなかった； BBBでのトランスポーター基質の透過、ならびにタイトジャンクションタンパク質のオクルディンとZO1の局在化と完全性も影響を受けなかった、と報告している。
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Investigation on the influence of a generic UMTS (Universal Mobile Telecommunications System) signal on barrier tightness, transport processes and the morphology of porcine brain microvascular endothelial cell cultures serving as an in vitro model of the blood-brain barrier.
Response of the cells to thermal stress was also investigated (with elevated temperatures of 37.3 - 39°C).
|ばく露時間||continuous for 1 up to 3.5 days|
|チャンバの詳細||Two radial waveguides each containing up to 28 samples were placed into an incubator and were used in turns as the exposure device or sham-exposure device. The samples in each waveguide were arranged symmetrically near the rim. The reflections were reduced by a 5-mm flat absorber along the waveguide perimeter.|
|ばく露装置の詳細||The filters carrying the cell cultures were installed in cylindrical tubes between two discoid gold electrodes. Each sample holder was equipped with a two-electrode system for impedance monitoring. The leads of the electrodes were conducted upward through the metal caps that sealed the cartridges. Thus simultaneous RF-field exposure and LF impedance measurement was possible.|
|Additional information||The barrier tightness was monitored continuously by TEER measurement using impedance spectroscopy described in detail by Wegener et al. in Brain Res. 853, 115-124 (2000). Impedance analysis was carried out in the frequency range from 1 Hz to 500 kHz applying a sinusoidal voltage of ~30 mV amplitude.|
No evidence of radiofrequency-field-induced disturbance of the function of the blood-brain barrier was found. After and during exposure, the tightness of the blood-brain barrier quantified by 14C-sucrose permeation as well as by trans-endothelial electrical resistance remained unchanged compared to sham-exposed cultures. Permeation of transporter substrates at the blood-brain barrier as well as the localization and integrity of the tight junction proteins occludin and ZO1 were not affected either.