研究のタイプ: 医学／生物学の研究 (experimental study)

[痙攣閾値下の用量のピクロトキシンで前処置されたラットにおいてGSM放射は発作を誘発し、脳のc-Fos陽性を増加させる] med./bio.

GSM radiation triggers seizures and increases cerebral c-Fos positivity in rats pretreated with subconvulsive doses of picrotoxin

著者: Lopez-Martin E, Relova-Quinteiro JL, Gallego-Gomez R, Peleteiro-Fernandez M, Jorge-Barreiro FJ, Ares-Pena FJ
掲載誌: Neurosci Lett 2006; 398 (1-2): 139-144
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この研究は、発作を起こしやすい傾向を持つように作成された動物を用いて、脳活動に対する携帯電話様の電磁放射の影響を調べた。ピクロトキシンのけいれん閾値下用量の急性投与により発作傾向のある実験モデルに変換されたラットに、携帯電話と同様の強度で、2時間のGSM変調900 MHz電磁放射へのばく露を与えると、発作が誘発され、新皮質、古皮質、海馬、視床の神経活動マーカであるc-Fosのレベルが著しく上昇した。同様のピクロトキシン処理ラットでも無ばく露の場合は、発作を起こさず、脳のc-Fos数は有意に低かった。ピクロトキシンで前処理されていないラットにおいては、ばく露、無ばく露によってそのような差異は起こさなかった、と報告している。

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研究目的（著者による）

To study the effects of 2 h mobile phone irradiation (GSM) on rats made seizure-prone by injection of a subconvulsive dose of the GABA antagonist picrotoxin.

詳細情報

The effects were evaluated by observation of whether the rats suffered seizures and post mortem by immunochemical testing of relevant brain areas for c-Fos (a marker of neuronal activation that is produced in these brain areas by seizures).
4 groups of rats were invesigated (each of 7-8 rats): 1) Injection of picrotoxin (2 mg/kg) and exposure to irradiation, 2) injection of picrotoxin, 3) irradiation, 4) exposure to neither irradiation nor picrotoxin.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h
  • 電力: 0.225 W
  • SAR: 0.24 W/kg mean (1 g) (whole body)
  • SAR: 1.3 W/kg peak (1 g) (whole body)
  • SAR: 0.42 W/kg mean (1 g) (brain)
  • SAR: 0.47 W/kg peak (1 g) (brain)
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h
Picrotoxin-treated
  • 電力: 0.225 W
  • SAR: 0.15 W/kg mean (1 g) (whole body)
  • SAR: 0.85 W/kg peak (1 g) (whole body)
  • SAR: 0.27 W/kg mean (1 g) (brain)
  • SAR: 0.31 W/kg peak (1 g) (brain)

General information

Four groups of rats were given the following treatments: injection of picrotoxin, exposure to radiation, picrotoxin and radiation, neither picrotoxin nor radiation.

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM-modulated
ばく露装置
ばく露の発生源／構造
  • antenna, signal generator
チャンバの詳細 Rats were individually immobilised in a tube and placed in a irradiation cage previously calibrated for measurement of the irradiation absorbed.
ばく露装置の詳細 The rat was located between a irradiating antenna and a receiving antenna with its head at an EMF maximum (as determined by FDTD calculations for the setup).
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.225 W - - - -
SAR 0.24 W/kg mean 測定値および計算値 1 g whole body
SAR 1.3 W/kg peak 測定値および計算値 1 g whole body
SAR 0.42 W/kg mean 測定値および計算値 1 g brain
SAR 0.47 W/kg peak 測定値および計算値 1 g brain
測定および計算の詳細

The power absorbed by the rat was calculated as the difference between the powers dissipated in the cage with and without the rat in the tube. In order to calculate the SAR values, the absorbance was scaled using the values obtained in FDTD simulations of a phantom rat.

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Additional information Picrotoxin-treated
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM-modulated
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Additional information Picrotoxin was injected i.p. immediately before exposure.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.225 W - - - -
SAR 0.15 W/kg mean 測定値および計算値 1 g whole body
SAR 0.85 W/kg peak 測定値および計算値 1 g whole body
SAR 0.27 W/kg mean 測定値および計算値 1 g brain
SAR 0.31 W/kg peak 測定値および計算値 1 g brain

Reference articles

  • Fritze K et al. (1997): [ラット脳のゲノム反応に対する携帯電話通信マイクロ波ばく露によるグローバルシステムの影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

Data of group 1 (picrotoxin and irradiation) showed that rats suffered seizures and the levels of the neuronal activity marker c-Fos in neocortex, paleocortex, hippocampus and thalamus increased markedly. Non-irradiated picrotoxin-treated animals did not suffer seizures, and their cerebral c-Fos counts were significantly lower. Irradiation caused no such differences in rats that had not been pretreated with picrotoxin.
The authors conclude that GSM-type irradiation can induce seizures in rats following their facilitation by subconvulsive doses of picrotoxin, and that research should be pursued into the possibility that this kind of irradiation may similarly affect brain function in humans with epileptic disorders.

研究の種別:

研究助成

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