研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスでのペンチレンテトラゾール誘発性痙攣の閾値に対する携帯電話放射の影響] med./bio.

Effect of mobile phone radiation on pentylenetetrazole-induced seizure threshold in mice.

掲載誌: Iran J Basic Med Sci 2016; 19 (7): 800-803

この研究は、BALB/c雄マウスを、対照群急性ばく露群(携帯電話電磁界(217Hzパルス化した900-950 MHz)への30、60、90分間ばく露の単回ばく露)および慢性ばく露群(同様のばく露を30日間継続)に分け、痙攣閾値(痙攣誘発に要するペンチレンテトラゾール(PTZ)の静注投与量)への影響を調べた。その結果、急性ばく露群では痙攣閾値に変化がなかった;慢性ばく露群では強直性および間代性痙攣のどちらの閾値も低下し、かつ1回のばく露時間が長い方が低下の割合が大きかった、と報告している。

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研究目的(著者による)

The effects of acute and chronic exposures of mice to a 900-950 MHz electromagnetic field (GSM) on the risk for epileptic seizures should be investigated.

詳細情報

To induce epileptic seizure in the animal model, intravenous pentylenetetrazole infusion was used (5 mg/ml and 0.3 ml/min (remark EMF-Portal: or 0.5 ml/min? contradictory statements)). Each animal was observed throughout the infusion period and the duration between the start of infusion and onset of seizure stages was recorded and then converted to the threshold seizure dosage (i.e. mg of drug per kg of body weight). Sudden involuntary muscle jerk followed by rapid writhing movements of the head and neck and/or clonic movements of forelimbs were considered as the signs of clonic seizure, and spasmodic hind limb extension, extreme rigidity with forelimbs, and hindlimbs extended caudally were considered as the signs of tonic seizure, i.e. a seizure in which the musculature affected is permanently strongly tensed.
Male mice were divided into 7 groups (with at least n=8 animals per group): 1) acute exposure for 30 minutes, 2) acute exposure for 60 minutes, 3) acute exposure for 90 minutes, 4) chronic exposure for 30 minutes per day for 30 days, 5) chronic exposure for 60 minutes per day for 30 days, 6) chronic exposure for 90 minutes per day for 30 days, 7) control group (remark EMF-Portal: treatment of the control group and the chronology of exposures and epileptic seizure induction remain unclear).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900–950 MHz
Modulation type: pulsed
ばく露時間: continuous for 30 minutes
acute exposure
-
ばく露2: 900–950 MHz
Modulation type: pulsed
ばく露時間: continuous for 60 minutes
acute exposure
-
ばく露3: 900–950 MHz
Modulation type: pulsed
ばく露時間: continuous for 90 minutes
acute exposure
-
ばく露4: 900–950 MHz
Modulation type: pulsed
ばく露時間: intermittent for 30 minutes/day for 30 days
chronic exposure
-
ばく露5: 900–950 MHz
Modulation type: pulsed
ばく露時間: intermittent for 60 minutes/day for 30 days
chronic exposure
-
ばく露6: 900–950 MHz
Modulation type: pulsed
ばく露時間: intermittent for 90 minutes/day for 30 days
chronic exposure
-

ばく露1

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30 minutes
Additional information acute exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • セルラ-電話
チャンバの詳細 plastic cages (45 cm diameter, 11 cm height) (remark EMF-Portal: it is not clear how many animals were exposed simultaneously)
ばく露装置の詳細 cell phone in answering mode was positioned in a way that the maximum distance to the animals was about 10 cm
パラメータ

No parameters are specified for this exposure.

ばく露2

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 60 minutes
Additional information acute exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

ばく露3

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 90 minutes
Additional information acute exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

ばく露4

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 30 minutes/day for 30 days
Additional information chronic exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

ばく露5

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 60 minutes/day for 30 days
Additional information chronic exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

ばく露6

主たる特性
周波数 900–950 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 90 minutes/day for 30 days
Additional information chronic exposure
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

Reference articles

  • Lopez-Martin E et al. (2009): [パルス変調GSM放射の作用はピクロトキシン誘発発作傾向のあるラットモデルの皮質および皮質下領域における脳活動およびc-Fos発現における限局的変化を増加させる]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Animals with chronic exposures to the electromagnetic field (groups 4-6) showed significantly lower threshold doses for tonic and clonic seizures compared to the control group and exposures for 60 and 90 minutes per day (groups 5 and 6) showed significantly lower values than exposure for 30 minutes per day (group 4).
Animals with acute exposures to the electromagnetic field (groups 1-3) did not show any significant differences compared to the control group.
The authors conclude that chronic exposure of mice to a 900-950 MHz electromagnetic field (GSM) might increase the risk for epileptic seizures.

研究の種別:

研究助成

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