【目的】900MHz、90 V/mの電磁界によりヒト‐ハムスター・ハイブリッド（AL）細胞株に生じる紡錘体異常について詳細に調べること。【方法】ここに述べる実験装置を用いると、電磁界の電界成分と磁界成分のどちらか、あるいは両方が潜在的な紡錘体異常の実効性に関連するかを調べることが可能になる。生物システムばく露時に、TEMモード電磁波の電界成分と磁界成分は分離され、別々にばく露できる。この手法は電磁界の非熱影響の基礎メカニズムの理解を深める識見を与えるであろう。【結果】5つの異なるばく露条件において得られた紡錘体異常を伴う後期および終期の割合を、擬似ばく露条件の場合と統計学的に比較した結果、対照群と差異がなかったのは電磁界の磁界成分にばく露された細胞のみであった。45、90 V/mの電界強度の電磁界、および電磁界から分離された電界成分へのばく露は細胞周期の後期および終期における有意な紡錘体異常を誘導することを、今回の結果は示唆した。
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To study whether either the electrical (E field) and/or the magnetic field (H field) component of an electromagnetic field at 900 MHz can be associated with the effectiveness of the spindle-disturbing potential (as shown in a previous study, see Schrader et al. 2008).
Three experiments were performed: Two experiments were performed at room temperature (20-22°C) and a third experiment at 37°C.
Experiments were conducted in six groups: i) E = 90 V/m, E-field and H-field, unmodulated travelling wave ii) E = 45 V/m, E-field and H-field, unmodulated travelling wave iii) E = 90 V/m, E-field only, standing wave iv) E < 0.1 V/m, H-field only, standing wave v) E = 90 V/m, E-field and H-field, amplitude modulated travelling wave (GSM signal used) vi) sham exposure
|ばく露時間||continuous for 0.5 h|
|ばく露装置の詳細||by placing a movable short cut at the end of the waveguide the E- fields and H-fields were spatially separated in the waveguide when producing a standing wave; cells fixed in a monolayer on a microscope slide, positioned inside the waveguide below the septum|
|Sham exposure||A sham exposure was conducted.|
The data showed that only cells exposed to the magnetic field component of the electromagnetic field were not different from the control. The results have confirmed the spindle-acting potential of a radiofrequency exposure at 835 MHz in human-hamster hybrid cells, which was reported for a 0.5 h exposure at field strengths from 45 to 90 V/m (see Schrader et al. 2008 and Schmid and Schrader 2007). Moreover, both the earlier and present data sets have shown that the fractions of anaphases and telophases with spindle disturbances are twice as high at 90 V/m than at 45 V/m. The findings were independent of the exposure temperatures at room temperature or 37°C.
The data indicate that an exposure of cells to an electromagnetic field at electric field strengths of 45 and 90 V/m, as well as to the separated E component of the electromagnetic field, induce significant spindle disturbances in anaphases and telophases of the cell cycle.