研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットの肺および肝臓組織に電磁界ばく露により生じる組織損傷の抑制における亜鉛補給の役割] med./bio.

The role of zinc supplementation in the inhibition of tissue damage caused by exposure to electromagnetic field in rat lung and liver tissues.

掲載誌: Bratisl Lek Listy 2012; 113 (7): 400-403

この研究は、6か月間、1日おきに、50 Hz磁界の5分間ばく露を継続して受けたラットの肺および肝臓組織誘発された酸化損傷、およびそれに対する亜鉛補給の効果を調べた。24匹の成獣の雄のSprague-Dawleyラットを同数の3群に分けた。すなわち、対照群磁界ばく露群、磁界ばく露+亜鉛補給群である。6か月間の処置の終了時に全ラットを屠殺して肺および肝臓組織試料を採取し、MDAはTBARS法で、GSHはビウレット法で、亜鉛レベルは原子発光によりそれぞれ測定した。その結果、磁界ばく露群の肺および肝臓組織のMDAレベルは、対照群および磁界ばく露+亜鉛補給群のレベルより有意に高く、磁界ばく露+亜鉛補給群のレベルは対照群より有意に高かった;GSHレベルについては、磁界ばく露+亜鉛補給群が、対照群および磁界ばく露群より有意に高く、磁界ばく露群は対照群より有意に高かった;肝臓および肺組織亜鉛レベルは、磁界ばく露+亜鉛補給群で最も高く、磁界ばく露群で最も低かった、と報告している。

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研究目的(著者による)

To investigate the effects of zinc supplementation on the oxidative damage in lung and liver tissues of rats exposed to an extremely low frequency magnetic field.

詳細情報

Three groups of rats were examined (n=8 per group): 1.) control group, 2.) magnetic field exposure and 3.) magnetic field exposure + daily zinc supplementation (injection of 3 mg zinc per kg body weight for 6 month).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 5 min every other day during 6 months

General information

rats were divided into the following three groups: i) sham EMF exposure ii) EMF exposure iii) EMF exposure + exposure to 3 (mg/kg)/day zinc sulphate

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 5 min every other day during 6 months
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 rats kept in 35 cm x 25 cm x 25 cm polyvinyl chloride cages with a single turn of 0.3 mm diameter copper wire wound around them for generation of the magnetic field; mean temperature 22°C; relative humidity 55% to 60%
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 30 W - - - -
磁束密度 5 µT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the exposure group, the lipid peroxidation in the lung and liver was significantly increased compared to the control group. A supplementation of zinc significantly lowered the lipid peroxidation compared to the exposure group without zinc, but the level of lipid peroxidation remained significantly higher in comparison to the control group.
The level of glutathione was significantly increased in the lung and liver of exposed rats when compared to the control group. A further significant increase in the concentration of glutathione was observed in exposed rats with zinc supplementation when compared to the exposure group without zinc.
In the lung and liver tissues of exposed rats, the content of zinc was significantly decreased in comparison to the control group, while in the lung and liver tissues of exposed rats with zinc supplementation the zinc content was significantly increased.
The authors conclude that exposure of rats to extremely low frequency magnetic fields could induce tissue damages in the lung and liver and that zinc supplementation could attenuate these damages.

研究の種別:

研究助成

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