この研究は、キイロタマホコリカビ細胞に対する超低周波磁界（50 Hz、300 μT）の影響を、8種類のバイオアッセイを用いて調べた。A群（24時間の擬似ばく露）、B群（24時間の磁界ばく露）、C群（48時間の擬似ばく露）、D群（24時間のばく露後に24時間の擬似ばく露）を比較した。その結果、24時間の磁界ばく露は、細胞質分裂（植物細胞などが仕切り作って細胞質分裂すること）速度、偽コリンエステラーゼの存在と活性、熱ショックタンパク70の存在に影響を与えたが、カタラーゼとグルタチオネインペロキシダーゼの活性に変化は見られなかった；これらの変化は一過性であり、24時間ばく露後24時間擬似ばく露した場合、これら変化したパラメータの値は対照群のレベルにまで戻った、と報告している。
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To examine the possible stressful effects of an extremely low frequency electromagnetic field on the slime mold Dictyostelium discoideum.
Two experiments were performed. First, the cell growth was measured after different time points: 1.) 24 hours sham exposure, 2.) 24 hours exposure, 3.) 48 hours sham exposure and 4.) 24 hours exposure, following 24 hours sham exposure.
Secondly, protein expression and enzyme activity were examined after A) 24 hours of sham exposure, B) 24 hours exposure or C) 24 hours exposure following 24 hours sham exposure.
|ばく露時間||continuous 24 or 48 hours|
|ばく露装置の詳細||each coil was made of 30 turns, medium radius being 14 cm and the distance between the coils was 17 cm; flasks containing the cell culture were positioned parallel to the symmetry axis of the coils (vertical axis)|
|Sham exposure||A sham exposure was conducted.|
|Additional information||for the sham exposure, Helmholtz coils identical to the active ones were used; however, the current flowed for 15 turns in one direction, and for another 15 turns in the opposite direction|
In the first experiment, the cell growth of the 24 hours-exposed group (group 2) was significantly reduced in comparison to the other groups. No significant differences were observed between group 3 (48 hours sham exposure) and group 4 (24 hours exposure + 24 hours sham exposure).
In the second experiment, the enzyme activity and the protein expression of pseudocholinesterase were significantly increased in the 24 hours-exposed group (group B) compared to the other groups. Additionally, the protein expression of HSP70 was significantly increased in the 24 hours-exposed group (group B) in comparison to the other groups. No significant differences were seen between group A (24 hours sham exposure) and group C (24 hours exposure + 24 hours sham exposure). No significant changes for the enzyme activities of catalase and glutathione peroxidase were observed between the groups.
The authors conclude that exposure to an extremely low frequency electromagnetic field could affect the cell growth, the enzyme activity and protein expression of pseudolcholinesterase and the protein expression of HSP70 in the slime mold Dictyostelium discoideum and that these effects were transient.