研究のタイプ: 医学/生物学の研究 (experimental study)

[インビトロでのアラビカコーヒーノキの苗立ち期、増殖期、順化期への60 Hz正弦波磁界の影響] med./bio.

Effects of 60 Hz sinusoidal magnetic field on in vitro establishment, multiplication, and acclimatization phases of Coffea arabica seedlings.

掲載誌: Bioelectromagnetics 2014; 35 (6): 414-425

この研究は、インビトロで栽培中のアラビカコーヒーノキ(学名Coffea arabica)の苗の苗立ち、増殖、順化期に対する60 Hz正弦波磁界の影響を調べた。ばく露群には、60 Hz正弦波磁界(2 mT)を3分間与え、無ばく露対照群と比較した。その結果、ばく露群では、純光合成量(増殖期85.4%、順化期117.9%)および光合成色素含量(苗立ち期66.6%、増殖期79.9%、順化期43.8%)が有意に増加した;また、ばく露群ではRBCS1遺伝子発現増加、呼吸率の低下がみられた、と報告している。

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研究目的(著者による)

To investigate the effects of extremely low frequency magnetic fields on the photosynthetic activity of in vitro (micropropagation) coffee seedlings.

詳細情報

Coffee is an important commercial product. Micrpropagation presents an in vitro culture system to generate high quality coffee seedlings. After a six weeks establishment phase in a culture medium, the plants reach a multiplication phase usually having three pairs of leaves and a primary root at the end. Afterwards, in the acclimatization phase, plants are transferred to a pot with soil.
Seedlings were exposed or sham exposed either during establishment, multiplication, or acclimatization for 3 minutes. For each experimental group (exposure and sham exposure, respectively), 50 samples were used per replicate for all phases.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: 3 minutes

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 3 minutes
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 electromagnet was formed by two parallel coils (dimensions 50 x 38 x 4 cm); coils were connected in series and coupled to a signal generator of sinusoidal waveform
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - ± 0.01 mT

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In exposed seedlings, the photosynthesis rate was significantly decreased during the establishment phase in comparison to the sham exposed seedlings, while during multiplication and acclimatization phase the photosynthesis rate was significantly increased. The transpiration was significantly decreased during all three phases in exposed seedlings when compared to the sham exposed ones, while the concentration of chlorophyll a and b was significantly increased. Additionally, the gene expression of the ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit was increased in the exposed samples during the acclimatization phase compared to the sham exposure.
Scanning electron microscopy revealed significantly higher stomata ornamentation and thicker cuticulas during the establishment and multiplication phase in the exposed seedlings in comparison to the sham exposed seedlings as well as most condensed leaf parenchyma with abundant amyloplasts and xylem tubes with thicker walls. In the acclimatization phase the leaves of exposed seedlings showed an increased cuticular wax content and the xylem vessel thickness was significantly higher than in sham exposed plants.
The results indicate that extremely low frequency magnetic fields may improve the coffee seedling's quality by influencing the photosynthetic activity of the seedlings during the multiplication and acclimatization phase and increasing their vigor.

研究の種別:

研究助成

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