研究のタイプ: 医学/生物学の研究 (experimental study)

[インビトロで繁殖させたコーヒーノキ(Coffea arabica L.)の生物学的パラメータに対する電磁界の影響] med./bio.

Effects of EMFs on Some Biological Parameters in Coffee Plants (Coffea arabica L.) Obtained by in vitro Propagation.

掲載誌: Pol J Environ Stud 2014; 23 (1): 95-101

この研究は、インビトロで繁殖中のコーヒーノキ(Coffea arabica L.)の苗木をソレノイドコイル(40cm)の内部に置き、60Hz、2mTの磁界ばく露の影響を調べた。活着期または増殖期の1週間後、あるいは移植後(順応期)に3分間の磁界ばく露を行った。50個の胚芽、50本の植物体での実験を3回繰り返した。その結果、それぞれの期での磁界ばく露群で、対照群に比べ、シュートおよび根の長さ、葉対の数が増加した、などを報告している。

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研究目的(著者による)

To investigate the possible effect of exposure to extremely low frequency magnetic fields on growth and antioxidative enzymes in in vitro (micropropagation) coffee seedlings and subsequent development of these seedlings.

詳細情報

Coffee is an important commercial product. Micrpropagation presents an in vitro culture system to generate high quality coffee seedlings. After a six weeks establishment phase in a culture medium, the plants reach a multiplication phase usually having three pairs of leaves and a primary root at the end. Afterwards, in the acclimatization phase, plants are transferred to a pot with soil.
Seedlings were exposed or sham exposed either during establishment, multiplication, or acclimatization for 3 minutes. For each experimental group (exposure and sham exposure, respectively), 50 samples were used per replicate and 3 replicates for all phases. Seedlings were examined at the end of the each phase or 4 month after the acclimatization phase,

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: 3 minutes

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 3 minutes
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 samples were placed in a dielectric container and placed in the homogeneous magnetic field at the end and center of the solenoid; a 40 cm long magnetizer solenoid was used, consisting of a 16-gauge copper wire (1.1 mm) with a total of 363 turns
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In exposed seedlings, the shoot and root lengths as well as leaf pair numbers were significantly increased during establishment, multiplication, or acclimatization phase and 4 month after acclimatization in comparison to seedlings from the control group. Magnetic field treatment resulted in a significant decrease of the enzyme activity of the catalase during establishment, multiplication and acclimatization, of the superoxide dismutase during acclimatization and of the ascorbate peroxidase during multiplication, acclimatization and in the 4 month old seedling compared to the corresponding control group. However, the enzyme activity of the catalase was significantly increased in the exposed 4 month old seedlings compared to the 4 month old control seedlings.
The authors conclude that exposure to extremely low frequency magnetic fields could influence antioxidative enzymes in in vitro (micropropagation) coffee seedlings and could have a promoting effect on growth and development.

研究の種別:

研究助成

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