研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界は根切り虫ハスモンヨウトウ幼虫の条件付けなしの動きを増加させる:磁界感受性に関する神経行動学的研究の新しいモデル] med./bio.

An extremely low frequency magnetic field increases unconditioned larval movement of the common cutworm, Spodoptera litura: A novel model for a magnetoreceptive neurobehavioral study.

掲載誌: Entomol Res 2008; 38 (4): 299-302

この研究は、動物磁界MF)への反応において最も関心が持たれるMF感受から行動への信号変換メカニズムを研究するため、よく見かける根切り虫ハスモンヨウトウを用いた行動学的アッセイ予備的研究を行った。その結果、擬似ばく露群に比べ、MF(60Hz、0.2 mT)連続ばく露群では、第3齢幼虫の動きが有意に増加した;擬似ばく露群に比べ、MF誘導性の動きは全継続時間が長く、開始も早かった;実験操作として単純で、条件付け無しの反応性を指標とする点で実験モデルに適すると考える、と報告している。

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研究目的(著者による)

To develop a model system for understanding the process of magnetoreceptive neurological behavioral in animals.

詳細情報

A preliminary behavioral assay was performed using the common cutworm (Spodoptera litura) to examine the magnetoreceptive behavior during exposure to an extremely low frequency magnetic field.
After an acclimatization period of several days, larvae (caterpillar) were placed in a round plastic Petri dish as a group (n=16 sham exposed, n=16 exposed) or as individuals (n=16 sham exposed, n=30 exposed).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露装置
ばく露の発生源/構造
チャンバの詳細 larvae were exposed in round plastic petri dishes (groups: diameter of 100 mm, individuals: diameter of 35 mm) which were put at the center of the testing arena (13 cm wide Œ 13 cm deep Œ 15 cm high) in the middle of the exposure system
ばく露装置の詳細 self-made exposure system (28 cm long Œ 28 cm deep Œ 30 cm high) was composed of open acrylic frames with three bundles (2000 turns per bundle) of copper wires (0.5 mm diameter) aligned parallel 20 cm apart and was connected to a locally supplied voltage transformer; temperature inside the exposure system was regulated (25°C ± 0.1°C)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.2 mT - 測定値 - -

Reference articles

  • Koh EK et al. (2008): [60Hz正弦波磁界は活性酸素種を介して前立腺がん細胞のアポトーシスを誘導する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中

研究の主なアウトカム(著者による)

Exposed larvae (group and individual) showed a significantly increased motor activity in comparison to the sham exposed larvae. The magnetic field-induced movement was significantly longer and had an earlier onset when compared to the sham exposure. In the exposed groups of larvae the onset time of movement was earlier than in the exposed individuals, maybe because of physical contact to other larvae, suggesting that the individual test is more favorable.
The authors conclude that investigating the motor behavior of the common cutworm (Spodoptera litura) while exposed to an extremely low frequency field represents a suitable model system in some areas of magnetoreceptive neurological behavioral research.

研究の種別:

研究助成

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