研究のタイプ: 医学/生物学の研究 (experimental study)

[細菌細胞での薬剤の効力に対する超低周波磁界の影響] med./bio.

Effect of Extremely Low-Frequency (ELF) Magnetic Fields on the Potency of Drugs in Bacterial Cells.

掲載誌: IEEE Trans Magn 2014; 50 (4): 1-4

この研究は、大腸菌への薬剤(5種類)の効力に対する超低周波磁界(60Hz、50mT)の影響を調べた。調べた薬剤は、分子量および細胞作用メカニズムなどがそれぞれ異なるミトキサントロン、ダウノルビシン、アクチノマイシンD、ブレオマイシン、およびジノスタチンである。その結果、ミトキサントロン、ダウノルビシン、アクチノマイシンD、ブレオマイシンは、6時間の磁界ばく露において、薬剤濃度にして約1.3倍の効力増強が見られたが、ジノスタチンでの効力増強は1.03倍であった(ジノスタチンの分子量は他の薬剤より10倍以上大きい);別に、マイトマイシンCで磁界周波数を変化させる実験を行ったが、最も有効な周波数は60Hzであった、と報告している。

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研究目的(著者による)

The effects of six cytostatics on the cell viability of Escherichia coli with and without exposure to a 60 Hz magnetic field should be investigated. Additionally, the influence of different field frequencies should be tested in this respect.

詳細情報

Each experiment was replicated six or seven times. The drugs tested were mitoxantrone, daunomycin, actinomycin D, bleomycin, zinostatin and mitomycin C. These were chosen because of their different molecular masses and modes of action.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 6 h
ばく露2: 20–280 Hz
ばく露時間: continuous for 6 h

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 6 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 the temperature within the experimental area was controlled by circulating water at 36°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 50 mT - 測定値 - -

ばく露2

主たる特性
周波数 20–280 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 6 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 50 mT - 測定値 - only in test with mitomycin C

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

In the experiments with mitoxantrone, daunomycin, actinomycin D and bleomycin, a distinct and significant decrease of viable cells could be observed in the approaches with exposure to the magnetic field and to the drug compared to approaches without the magnetic field. This was interpreted as an enhancement of the effects of the drugs with enhancements of ca. 30%, depending on the drug. The approaches with zinostatin, however, showed only an enhancement of the effects of 3% when exposed to the magnetic field simultaneously. The experiments on the influence of the field frequency on the effects of mitomycin C showed the strongest effect at 60 Hz.
The authors conclude that 60 Hz magnetic fields increase the cytostatic effects of the examined drugs on E. coli and that the magnitude of this increase depends on the frequency of the field and the molecular mass of the drug.

研究の種別:

研究助成

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