研究のタイプ: 医学/生物学の研究 (experimental study)

[大腸菌の細胞増殖および表面形態に対する塩化鉄と組み合わせたELF磁界の影響] med./bio.

Effects of ELF Magnetic Field in Combination with Iron(III) Chloride (FeCl3) on Cellular Growth and Surface Morphology of Escherichia coli (E. coli).

掲載誌: Appl Biochem Biotechnol 2013; 169 (8): 2341-2349

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研究目的(著者による)

To examine the effects of 50 Hz magnetic fields alone or in combination with FeCl3 on the cell viability and morphology of Escherichia coli.

詳細情報

Escherichia coli cells were grown in culture medium without or with different concentrations of iron(III) chloride (0.2, 2 and 20 g/l FeCl3) while being exposed or sham exposed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 30 ml plastic culture flasks containing E. coli in a fluid medium were used
ばく露装置の詳細 coils were made up of 154 turns of copper wire with a diameter of 2.2 mm having inner and outer diameters of 40.5 and 45.5 cm, respectively; coils were placed in horizontal plane to produce vertically oriented magnetic field; distance between the coils was 21.375 cm; electrical parameters of the coils were resistance of 1.2 ohm and inductance of 19.6 mH; coils were driven with a variable transformer with a power of 2.7 kVA; system was mounted on a wooden frame; culture flasks were placed at the center of the coil-based exposure system (homogenous exposure region) in vertical position, so that the magnetic field was perpendicular to the bottom of the flasks
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - ± 0.05 mT
電流密度 69.3 mA/m² maximum - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences between exposed and sham exposed cells were observed regarding cell viability. Furthermore, no significant influence of FeCl3 supplementation of the culture medium on the cell viability was found.
While the morphology of sham exposed cells appeared normal in the scanning microscopy, exposed cells showed structural damages such as various pores with different sizes on the cell membrane.
The authors conclude that 50 Hz magnetic fields do not influence the cell viability of Escherichia coli but could affect the surface morphology.

研究の種別:

研究助成

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