この研究は、不死化ヒト気管支上皮細胞（BEAS-2B細胞株）の培養系を用いて、創傷の治癒に対する連続電磁界（EMF：1.8GHz、SARは1.0W/kg）の効果を調べた。EMFの効果は、色素MTTを用いた細胞死アッセイ、細胞数カウント、蛍光標識による細胞分裂周期別の細胞分類（FACS）、スクラッチアッセイ（培養表面にひっかき傷を付ける）での細胞移動で評価した。その結果、EMF群では対照群に比べ、スクラッチアッセイでの細胞移動が低下した；MTTアッセイ、細胞数カウントによれば、EMF処置は細胞毒性を示さず、細胞増殖を抑制しなかった；細胞分裂周期分布に変化は見られなかった、と報告している。
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The effects of an in vitro exposure of human bronchial epithelial cells to a 1.8 GHz electromagnetic field on cell proliferation and migration in view of wound healing should be investigated.
The scratch assay is a simple test to measure cell migration. In this assay, a thin "wound" is introduced into a surface with a dense cell growth by scratching with a pipette tip. Cells on the wound edge subsequently migrate into the wound space until the wound is closed. The scratch assay was performed either during a continuous exposure of up to 65 h or under the conditions of an exposure which was canceled after 24 hours.
All experiments were repeated at least twice.
| 周波数 | 1.8 GHz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for up to 65 h |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | cells were exposed in plates with wells |
| ばく露装置の詳細 | six well plates were exposed simultaneously; cells were in close contact above antenna in H-field maximum; temperature, humidity and SAR were controlled during exposure; cells were maintained under a humidified atmosphere of 5% CO2 and 95% air at 37°C |
| Sham exposure | A sham exposure was conducted. |
Neither cell viability nor cell proliferation, cell cycle distribution or apoptosis were significantly changed in exposed cells compared to the sham exposure.
The wound healing rate was significantly reduced in exposed cells from 6 to 54 hours and finally, exposed cells needed 65 hours for a complete restoration of the cell layer in comparison to 48 hours in sham exposed cells. In the approach with an exposure solely during the first 24 hours, the healing rate was significantly lower in exposed cultures from 3 to 48 hours compared to the sham exposure. Finally, exposed cells needed 55 hours and sham exposure 48 hours for a complete healing of the scratch. However, the healing rate of cells in this approach was significantly higher from 36 to 48 hours than in the continuously exposed cells.
The authors conclude that an in vitro exposure of human bronchial epithelial cells to a 1.8 GHz electromagnetic field could reduce the wound healing capacity in cell cultures due to an impaired cell migration.
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