研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界ばく露下の線虫(Caenorhabditis elegans)におけるエネルギー代謝] med./bio.

The energy metabolism in Caenorhabditis elegans under the extremely low-frequency electromagnetic field exposure.

掲載誌: Sci Rep 2015; 5: 8471

この研究は、50Hzの超低周波磁界(ELF-EMF磁界強度0.5、1、2、3mT、ばく露時間48時間、3mTのみ30日間)ばく露下に置かれた線虫(Caenorhabditis elegans)の代謝物をガスクロマトグラフ-飛行時間型質量分析計(GC-Tof/MS)で分析した。その結果、代謝物の小さな変動が見られたが、一定のパターンは観察されなかった;エネルギー代謝に関連するピルビン酸、フマール酸、L−リンゴ酸は、全てのばく露群で上昇した、などの所見を報告している。

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研究目的(著者による)

The effects of exposure of Caenorhabditis elegans to a 50 Hz magnetic field on the energy metabolism should be investigated.

詳細情報

Energy metabolism enhancement is a known adaptive response to stress situations and Caenorhabditis elegans is a typical model organism to study the effects of environmental influences on biological systems.
Eggs and worms were either exposed to 0.5 mT (group 1), 1 mT (group 2), 2 mT (group 3), 3 mT (group 4) or not exposed (control group). All results were obtained from at least 3 replicates of the tests.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 48 h
ばく露2: 50 Hz
ばく露時間: continuous for 48 h
ばく露3: 50 Hz
ばく露時間: continuous for 48 h
ばく露4: 50 Hz
ばく露時間: continuous for up to 30 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 48 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two vertical Helmholtz coils (150 turns of copper wire with diameter of 1 mm) in an incubator; culture dishes were placed in the center of the coils; temperature was recorded during exposure (temperature level not specified)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 48 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 48 h
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for up to 30 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The tests on hatching rate and brood size showed no significant differences between exposure group 4 and the control group.
The amount of single metabolites was significantly increased in different exposure groups compared to the control group, without showing a regular trend with increasing magnetic flux densities or consistent results in the exposure groups. The gene expression of glyceraldehyde phosphate dehydrogenase-1 and -4, the GAPDH level and the enzyme activity were significantly increased in exposure group 4 compared to the control group, whereas the ATP level did not show any significant differences.
The food consumption was significantly decreased in exposure group 4 compared to the control group, while the mortality was not changed significantly.
The authors conclude that exposure of Caenorhabditis elegans to a 50 Hz magnetic field could enhance the energy metabolism while restricting the food intake.

研究の種別:

研究助成

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