研究のタイプ: 医学/生物学の研究 (experimental study)

[トウモロコシ(Zea mays)芽苗の初期成長のEMF放射(1800MHz)による抑制にはデンプンおよびショ糖の代謝変化が関与する] med./bio.

EMF radiations (1800 MHz)-inhibited early seedling growth of maize (Zea mays) involves alterations in starch and sucrose metabolism.

掲載誌: Protoplasma 2016; 253 (4): 1043-1049

この研究は、トウモロコシ(Zea mays)芽苗の成長およびデンプン、ショ糖、リン加水分解酵素の活性に対する1800MHz電磁界放射EMF)の影響を調べた。トウモロコシ芽苗にばく露したEMFは、一様な変調連続波で、SARは0.169W/kgである。0.5、1、2、4時間のばく露を行い、その7日後の芽苗を分析した。その結果、短時間のばく露は何ら有意な変化を引き起こさなかったが、4時間ばく露は成長および生化学的指標に有意な変化を引き起こした;具体的には、根および子葉鞘の長さの短縮、特に子葉鞘長は23%と顕著な短縮が見られた;無ばく露群に比べ、4時間ばく露群では光合成色素量が13%、総炭水化物量が18%減少した;同じくデンプン加水分解酵素のα-およびβ-アミラーゼ活性が無ばく露群よりそれぞれ92および94%上昇した、などを報告している。

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研究目的(著者による)

The effects of exposure of maize seedlings to a 1800 MHz electromagnetic field on growth and the carbohydrate metabolism should be investigated.

詳細情報

After germination, seedlings were divided into the following groups: exposure to the electromagnetic field for 1) 30 minutes, 2) 1 hour, 3) 2 hours, 4) 4 hours and 5) no exposure (control group). Each group consisted of 5 petri dishes with 10 seedlings each (n=50 seedlings in total). All seedlings were further cultivated after exposure and investigated after 7 days.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 30 minutes
ばく露2: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 1 hour
ばく露3: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 2 hours
ばく露4: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 4 hours

General information

SAR calculations based on Andreuccetti D, Fossi R, Petrucci C (1997) An internet resource for the calculation of the dielectric properties of body tissues in the frequency range 10 Hz-100 GHz. http://niremf.ifac.cnr.it/tissprop/, (Italy): IFAC-CNR; Based on data published by C Gabriel et al. in 1996

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30 minutes
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • antenna
Distance between measurement device and exposure object 3 cm
チャンバの詳細 petri dishes (15 cm diameter)
Additional information exposure system was kept in an empty separate room coated with special radiofrequency shielding paint in order to protect from external exposure sources
パラメータ
測定量 種別 Method Mass 備考
電力密度 332 mW/m² mean 測定値 - ± 10.36 mW/m2
電力 0.1 W - - - -
SAR 1.69 W/kg - 計算値 - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 hour
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between measurement device and exposure object 3 cm
パラメータ
測定量 種別 Method Mass 備考
電力密度 332 mW/m² mean 測定値 - ± 10.36 mW/m2
電力 0.1 W - - - -
SAR 1.69 W/kg - 計算値 - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 hours
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between measurement device and exposure object 3 cm
パラメータ
測定量 種別 Method Mass 備考
電力密度 332 mW/m² mean 測定値 - ± 10.36 mW/m2
電力 0.1 W - - - -
SAR 1.69 W/kg - 計算値 - -

ばく露4

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 4 hours
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between measurement device and exposure object 3 cm
パラメータ
測定量 種別 Method Mass 備考
電力密度 332 mW/m² mean 測定値 - ± 10.36 mW/m2
電力 0.1 W - - - -
SAR 1.69 W/kg - 計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After 4 hours of exposure, the length of roots and coleoptiles as well as the chlorophyll content were significantly reduced compared to the control group (group 5).
The carbohydrate and reducing sugar contents were significantly increased after 1 hour of exposure or more (groups 2, 3 and 4) compared to the control group.
The enzyme activities of α-amylase, β-amylases and invertases were significantly increased in groups 2,3 and 4 compared to the control group, whereas the enzyme activity of the starch phosphorylases was significantly decreased at the same time. No distinct trend was observed in the enzyme activities of the phosphatases.
In all parameters, a tendency towards enhanced effects with increasing exposure duration was observed.
The authors conclude that exposure of maize seedlings to a 1800 MHz electromagnetic field could interfere with growth and the carbohydrate metabolism.

研究の種別:

研究助成

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