研究のタイプ: 医学/生物学の研究 (experimental study)

[全脳虚血されたスナネズミにおける海馬ニューロンおよびグリア細胞の交流磁界への応答] med./bio.

Response of hippocampal neurons and glial cells to alternating magnetic field in gerbils submitted to global cerebral ischemia.

掲載誌: Neurotox Res 2013; 23 (1): 79-91

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研究目的(著者による)

To investigate whether exposure to an extremely low-frequency magnetic field affects the outcome of post-ischemic damage in the hippocampus of Mongolian gerbils.

詳細情報

Cerebral ischemia was induced by surgical occlusion of both common carotid arteries for 10 min. Adult (3-month-old) male gerbils were divided into six groups: 1.) intact controls (n=3), 2.) sham operated (n=6), 3.) sham exposure (n=6), 4.) exposure (n=9), 5.) ischemia + sham exposure (n=9), and 6.) ischemia + exposure (n=9). All groups were subdivided into two groups and histological analyses were performed immediately at the end of exposure (on day 7) and 7 days after cessation of exposure (on day 14), respectively.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 7 days

General information

Animals were divided into the following groups: i) intact controls ii) sham operated iii) sham exposure iv) exposure v) ischemia + sham exposure vi) ischemia + exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7 days
ばく露装置
ばく露の発生源/構造
  • electromagnet
ばく露装置の詳細 two 26 cm x 43 cm x 15 cm cages with 3 - 4 animals placed at either side of the electromagnet so that the cage's center was at 20 cm distance from it
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT maximum 測定値 - 0.2 - 2.0 mT
磁束密度 0.5 mT average over time 測定値 - at the cage's center

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to extremely low frequency magnetic fields alone did not induce any morphological changes in the hippocampus, while 10-min global cerebral ischemia led to neuronal death, especially in the CA1 region of the hippocampus.
Ischemic gerbils exposed to extremely low frequency magnetic fields (group 6) had a significantly lower degree of cell loss in the hippocampus and greater responses of astrocytes and microglial cells than post-ischemic gerbils with shamexposure (group 5) at the end of the 7-day exposure period. A similar response was observed on the seventh day after cessation of exposure (day 14, delayed effect); however, differences were low and the results statistically non-significant.
The authors conclude that exposure to extremely low frequency magnetic fields has possible neuroprotective function in the hippocampus, as the most sensitive brain structure in the model of global cerebral ischemia, through reduction of neuronal death and activation of astrocytes and microglial cells.

研究の種別:

研究助成

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