この研究は、DNA合成および細胞増殖のために必要なポリアミン合成の律速酵素であるオルニチンデカルボキシラーゼ（ODC：全ての有核細胞に存在する）に対する正弦波振幅変調マイクロ波ばく露の影響を、Reuber H35肝細胞腫細胞を用いて調べた。マイクロ波の搬送周波数は450 MHz、変調周波数は16、60、100 Hzとした。ピーク電力密度は1.0mW / cm2であり、非熱的レベル（温度上昇が0.1℃未満）であったとしている。その結果、変調周波数16 Hzで1時間ばく露の場合、ODC活性が約50％上昇し、上昇した活性レベルはばく露後数時間続いた；変調周波数60Hzおよび100Hzでの1時間ばく露の場合、ODC活性に変化は見られなかった、などの知見を報告している。
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To study the ornithine decarboxylase activity in cultured cells exposed to low energy modulated microwave fields and phorbol ester tumor promotors.
Ornithine decarboxylase activity is regulated by a wide variety of growth factors and hormones active on the cell surface. Cyclic AMP and phorbol ester tumor promotors, in addition to other membrane-active compounds, have been shown to rapidly modulate ornithine decarboxylase activity. The tumor-promoting phorbol esters, including TPA used in this study, have a specific membrane receptor, the calcium-phospholipid kinase (protein kinase C). Since latent cell transformation manifested in the presence of phorbol esters has been reported following low-level microwave exposure, ornithine decarboxylase might be a sensitive molecular marker of field-related actions related to the process of tumor promotion.
| 周波数 | 450 MHz |
|---|---|
| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 1 h |
| Modulation type | AM |
|---|---|
| Modulation frequency | 16 Hz |
| Modulation depth | 85 % |
| Additional information |
sinusoidally modulated at 75-85% |
| ばく露の発生源／構造 |
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|---|---|
| チャンバの詳細 | The long axis of the Crawford cell was oriented vertically inside a large incubator at 37 ± 0.5°C. |
| ばく露装置の詳細 | Cell cultures of 10 ml in circular Petri dishes (75 cm²) were placed in a gas-tight Lucite box inside the Crawford cell near the center of the long axis at a distance of 1 cm from each other. Air containing 5% CO2 at 100% humidity was passed continuously into the Lucite culture chambers. |
| Sham exposure | A sham exposure was conducted. |
| Additional information | Control cultures were placed in the same incubator in a similar box outside the Crawford cell. |
| 周波数 | 450 MHz |
|---|---|
| タイプ |
|
| 特性 |
|
| ばく露時間 | continuous for 1 h |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
In all three cell types, a 1-h radiation produced a significant (up to 50%) increase in ornithine decarboxylase activity in comparison to the unexposed cultures. Following removal from the field, ornithine decarboxylase activity remained elevated in the exposed cells for more than a 3-h period in the Reuber H35 and CHO cells (the increase in the 294T cells persisted for only 1 h).
Modulation frequencies of 60 and 100 Hz failed to cause any alteration in ornithine decarboxylase activity in Reuber H35 cells following a 1-h radiation, in contrast to the 50% increase in enzyme activity brought about the 16 Hz modulated field.
The stimulated ornithine decarboxylase activity in the cultured cells that followed treatment with a phorbol ester tumor promotor (TPA) was potentiated by prior exposure to the same low energy electromagnetic field.The field did not alter either basal or TPA-stimulated DNA synthesis.
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