研究のタイプ: 医学／生物学の研究 (experimental study)

[2.45 GHzマイクロ波とホルボールエステルによる C3H/10T[1/2] 細胞での悪性形質転換の誘導] med./bio.

Induction of neoplastic transformation in C3H/10T1/2 cells by 2.45 GHz microwaves and phorbol ester

著者: Balcer-Kubiczek EK, Harrison GH
掲載誌: Radiat Res 1989; 117 (3): 531-537
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この研究は、3.45Gy/分で1.5Gyの238kVp X線照射および／または24時間の2.45GHzマイクロ波ばく露の処理を終えたC3H/10T1/2細胞に、腫瘍プロモーターであるテトラデカノイル - ホルボール-13-アセテート（TPA）0.1 µg/ mlを投与して培養した場合と投与無しで培養した場合について、形質転換頻度および細胞生存率を比較した。その結果、TPA無しで培養した場合の形質転換反応はX線照射単独の場合と同等であった；TPAによる形質転換応答の増強は、2.45GHzマイクロ波（SAR = 4.4W / kg）ばく露を受けた細胞において有意に大きかった；マイクロ波単独ばく露の場合、形質転換への影響は見られなかった；平板培養効率および細胞生存力に対してはTPA処置またはマイクロ波ばく露の影響は見られなかった、と報告している。

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研究目的(acc. to editor)

To study transformation frequency and cell survival with or without postirradiation addition of the tumor promoter TPA in C3H 10T1/2 cells exposed to 2.45 GHz microwaves and/or X-rays.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 24 h
  • 電力: 1 kW peak (forward power to the antenna)
  • SAR: 4.4 W/kg mean (± 0.8 W/kg)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 24 h
Modulation
Modulation type pulsed
Pulse width 83 µs
Repetition frequency 120 Hz
ばく露装置
ばく露の発生源／構造
  • quarter-wave transformer
チャンバの詳細 The exposure facility has previously been described in detail [Harrison et al., 1985].
ばく露装置の詳細 Three 25-cm² cell-culture flasks filled with medium were placed horizontally and cell-side down beneath a quarter-wave transformer covering the central 25 x 25 cm region of a large (60 x 60 x 12 cm) constant-temperature (37.2 ± 0.1°C) water bath with their cellular attachment plane approx. 3 cm below water level. Two flasks for sham exposure were placed inside a grounded copper-mesh enclosure located at the bottom, peripheral to the central region.
Sham exposure A sham exposure was conducted.
Additional information Cells were exposed to MW only, or they were pre- or post-irradiated with 1.5 Gy of 238-kVp X rays. Additional groups were sham exposed or exposed to X rays only. It is not really clear from the article whether or when the latter group was also sham exposed.
パラメータ
測定量 値 種別 Method Mass 備考
電力 1 kW peak 測定値 - forward power to the antenna
SAR 4.4 W/kg mean 測定値 - ± 0.8 W/kg
測定および計算の詳細

The bulk temperature in the flasks and forward power to the antenna were monitored continuously. The SAR at the cell monolayer has been determined previously from calorimetric and field measurements using a three-dimensional EF probe [Harrison et al., 1985].

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A statistically significant enhancement of transformation response by TPA in cells exposed to 2.45 GHz microwaves is shown. Microwaves alone had no effect on cell transformation. Plating efficiency and cell survival were not affected by TPA or microwaves.

研究の種別:

研究助成

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