研究のタイプ: 医学／生物学の研究 (experimental study)

[120Hz変調2.45GHｚマクロ波と腫瘍促進剤フォボルエステルへばく露後のC3H/10T1/2細胞の新生物形質転換] med./bio.

Neoplastic transformation of C3H/10T1/2 cells following exposure to 120-Hz modulated 2.45-GHz microwaves and phorbol ester tumor promoter

著者: Balcer-Kubiczek EK, Harrison GH
掲載誌: Radiat Res 1991; 126 (1): 65-72
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この研究は、盛んに増殖中のC3H/10T1/2培養細胞に、120Hzパルス変調2.45GHzマイクロ波ばく露（SAR：0.1、1、4.4 W/kg）を24時間与えた。別の実験として、4.4W/kgでの24時間ばく露の前または後にX線ばく露（0.5、1、1.5Gy）を与えた。これらの処理の後にTPA投与をした場合としない場合について、生存率および悪性形質転換のアッセイを行った。その結果、電磁界単独では、生存率および悪性形質転換誘発に影響は見られなかった；電磁界に加えてTPA投与をした場合、悪性形質転換は有意に増強され、1.5GyのX線と同等の影響が見られた、などを報告している。

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研究目的（著者による）

In a previous study (publication 862) a statistically significant enhancement of transformation response by TPA in cells exposed to 2.45 GHz microwaves was shown.
This study was intended to show that the enhancement of neoplastic transformation due to electromagnetic fields plus TPA is dependent on the level of electromagnetic radiation and is additive with doses of X-rays given as a cocarcinogen.

詳細情報

Actively growing cells were exposed for 24 h to 2.45-GHz microwaves with or without subsequent incubation in TPA (0.1 µg/ml). In separate experiments, a 24-h electromagnetic field exposure at 4.4 W/kg was preceded or followed by X-radiation at 0.5, 1, or 1.5 Gy (with or without subsequent incubation in TPA).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 24 h
  • 電界強度: 18 V/m peak (56 V/m and 120 V/m)
  • 磁束密度: 90 nT peak (0.27 µT and 0.56 µT)
  • SAR: 0.1 W/kg unspecified (cellular monolayers were placed 8.7 cm below water level)
  • SAR: 1 W/kg unspecified (cellular monolayers were placed 4.8 cm below water level)
  • SAR: 4.4 W/kg unspecified (cellular monolayers were placed 2.3 cm below water level)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 120 Hz
ばく露装置
ばく露の発生源／構造
  • magnetron
ばく露装置の詳細 Culture flasks immersed in water-bath. The cellular attachment plane was located more than 20 wavelengths away from the source and was positioned perpendicularly to the direction of EM wave propagation, parallel to EM field polarization plane. Shielded sham control flasks were located at the bottom of the water bath at a depth of 11 cm.
Additional information In a separate experiment, samples were exposed to 4.4 W/kg and were irradiated to x-ray prior or after the exposure at 0.5, 1 or 1.5 Gy.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 18 V/m peak 推定値 - 56 V/m and 120 V/m
磁束密度 90 nT peak 推定値 - 0.27 µT and 0.56 µT
SAR 0.1 W/kg unspecified 指定なし - cellular monolayers were placed 8.7 cm below water level
SAR 1 W/kg unspecified 指定なし - cellular monolayers were placed 4.8 cm below water level
SAR 4.4 W/kg unspecified 指定なし - cellular monolayers were placed 2.3 cm below water level

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

There were no significant effects of TPA or electromagnetic fields on plating efficiency, or on survival in combination with X-radiation.
The electromagnetic field or TPA exposure alone had no effect on induction of neoplastic transformation. However enhancement of transformation due to electromagnetic fields plus TPA was highly significant (synergistic dose-dependent effect). It ranged up to a level equivalent to that produced by 1.5 Gy of X-rays. The frequency of neoplastic transformation was dependent on the level of electromagnetic radiation and was additive with doses of X-rays given as a cocarcinogen.

研究の種別:

研究助成

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