The experiments on human keratinocytes were performed in triplicate.
In order to investigate whether a reduction in cell proliferation of exposed cells was due to the activation of a differentiation process, the expression of two markers of differentiation (keratin 1 and involucrin) were examined.
|Exposure duration||continuous for 18 h|
|Additional info||publication 14367: Weiland, T., 1977, A Discretization Method for the Solution of Maxwell's Equations for Six-Component Field, Electronics and Communications AEÜ 31(3), 116-120.|
|Chamber||A parallelepiped PVC container with front and rear sides open and hollow top and bottom walls passed through by thermostated water at 37°C was placed inside a GTEM chamber.|
|Setup||Three 25 cm² (T25) flasks with 5 ml of medium were placed in the central zone of the PVC container.|
|Sham exposure||A sham exposure was conducted.|
A reduction in the number of exposed keratinocytes in comparison to sham-exposed ones was found. However, the number of dead keratinocytes was not significantly different in exposed and sham-exposed samples.
Both keratin 1 and involucrin expression was increased in the exposed keratinocytes.
These results suggest that this protocol of exposure to 900 MHz electromagnetic field can trigger a differentiation program in keratinocytes.