Study type: Medical/biological study (experimental study)

In Vitro Evaluation of Biological Effects on Human Keratinocytes Exposed to 900 MHz Electromagnetic Field. med./bio.

Published in: Environmentalist 2005; 25 (2-4): 113-119

Aim of study (acc. to author)

To elucidate the effects of 900 MHz electromagnetic fields on the skin.

Background/further details

The experiments on human keratinocytes were performed in triplicate.
In order to investigate whether a reduction in cell proliferation of exposed cells was due to the activation of a differentiation process, the expression of two markers of differentiation (keratin 1 and involucrin) were examined.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 18 h

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Waveform
Charakteristic
  • guided field
Exposure duration continuous for 18 h
Additional info publication 14367: Weiland, T., 1977, A Discretization Method for the Solution of Maxwell's Equations for Six-Component Field, Electronics and Communications AEÜ 31(3), 116-120.
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber A parallelepiped PVC container with front and rear sides open and hollow top and bottom walls passed through by thermostated water at 37°C was placed inside a GTEM chamber.
Setup Three 25 cm² (T25) flasks with 5 ml of medium were placed in the central zone of the PVC container.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 50 V/m effective value - - -
SAR 0.08 W/kg mean calculated - 0.032-0.122 W/kg for 59% of cells in lateral samples
SAR 0.04 W/kg mean calculated - 0.015-0.065 W/kg for 73% of cells in central sample

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A reduction in the number of exposed keratinocytes in comparison to sham-exposed ones was found. However, the number of dead keratinocytes was not significantly different in exposed and sham-exposed samples.
Both keratin 1 and involucrin expression was increased in the exposed keratinocytes.
These results suggest that this protocol of exposure to 900 MHz electromagnetic field can trigger a differentiation program in keratinocytes.

Study character:

Study funded by

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