研究のタイプ: 医学／生物学の研究 (experimental study)

[900 MHz電磁界にばく露されたヒトケラトニサイトに対する生物効果のIn vitro評価] med./bio.

In Vitro Evaluation of Biological Effects on Human Keratinocytes Exposed to 900 MHz Electromagnetic Field

著者: Duranti G, Rossi A, Rosato N, Fazio G, Sacerdoti G, Rossi P, Falsaperla R, Cannelli V, Supino R
掲載誌: Environmentalist 2005; 25 (2-4): 113-119
RISフォーマットで参考文献をダウンロード

この研究は、ヒトのケラチノサイトに対する超高周波の電磁放射の生物学的影響を調べた。自然に不死化されたヒトケラチノサイト細胞株であるHaCaT細胞を実験に用いた。HaCaT細胞は、GTEMチャンバ内の平面波条件で、周波数900 MHzの正弦波電磁界（平均SARレベル：0.04から0.08 W / kg）に、温度は37°Cに制御された状態で、18時間ばく露を受けた。ばく露群および無ばく露対照群の細胞増殖曲線が得られた。結果として、対照群に比べ、ばく露群では細胞数が低下した；ただし、死んだ細胞の数には、ばく露群と対照群で有意差がなかった；ばく露群での細胞増殖低下の原因が分化過程の活性化にあるか否かを調べるため、分化のマーカー・タンパク質であるケラチン1およびインボルクリンの発現を測定したところ、２つの発現は、対照群に比べ、ばく露群で増加した、この知見は、この実験で用いた900 MHz電磁界ばく露プロトコルがHaCaT細胞の分化プログラムをトリガーできることを示す、と報告している。

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研究目的（著者による）

To elucidate the effects of 900 MHz electromagnetic fields on the skin.

詳細情報

The experiments on human keratinocytes were performed in triplicate.
In order to investigate whether a reduction in cell proliferation of exposed cells was due to the activation of a differentiation process, the expression of two markers of differentiation (keratin 1 and involucrin) were examined.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 18 h
  • 電界強度: 50 V/m effective value
  • SAR: 0.08 W/kg mean (0.032-0.122 W/kg for 59% of cells in lateral samples)
  • SAR: 0.04 W/kg mean (0.015-0.065 W/kg for 73% of cells in central sample)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
特性
  • guided field
ばく露時間 continuous for 18 h
Additional information publication 14367: Weiland, T., 1977, A Discretization Method for the Solution of Maxwell's Equations for Six-Component Field, Electronics and Communications AEÜ 31(3), 116-120.
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • GTEMセル
チャンバの詳細 A parallelepiped PVC container with front and rear sides open and hollow top and bottom walls passed through by thermostated water at 37°C was placed inside a GTEM chamber.
ばく露装置の詳細 Three 25 cm² (T25) flasks with 5 ml of medium were placed in the central zone of the PVC container.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 50 V/m effective value - - -
SAR 0.08 W/kg mean 計算値 - 0.032-0.122 W/kg for 59% of cells in lateral samples
SAR 0.04 W/kg mean 計算値 - 0.015-0.065 W/kg for 73% of cells in central sample
測定および計算の詳細

Numerical dosimetry was performed based on the Finite Integration Technique (FIT) [Weiland, 1977]. All materials were characterised on the basis of literature [Chou et al., 1996]. A linearly polarised plane wave was assumed incident on the PVC box with the EF orthogonal to the surface of the samples and the propagation direction parallel to the major side of the flasks. Local spatial and total average SARs for all samples were computed from EF values.

Reference articles

  • Chou CK et al. (1996): [無線周波電磁界ばく露：実験的ドシメトリに関する教育的レビュー]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A reduction in the number of exposed keratinocytes in comparison to sham-exposed ones was found. However, the number of dead keratinocytes was not significantly different in exposed and sham-exposed samples.
Both keratin 1 and involucrin expression was increased in the exposed keratinocytes.
These results suggest that this protocol of exposure to 900 MHz electromagnetic field can trigger a differentiation program in keratinocytes.

研究の種別:

研究助成

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