24 immature (two weeks old) and 24 mature (ten weeks old) rats were divided into six groups (each group n=8): 1) immature control group, 2) immature exposure group, 3) immature exposure group + recovery period of 15 days, 4) mature control group, 5) mature exposure group, 6) mature exposure group + recovery period of 15 days.
Bone marrow cells were collected from both femurs. Four rats were used for the micronucleus test and four rats for the chromosomal analysis and mitotic index.
Modulation type: pulsed
Exposure duration: continuous for two hours/day for 45 days
Rats were divided into six groups with eight animals each: i) immature control group ii) immature rats exposed to EMF iii) immature rats granted a recovery period of 15 days after exposure termination iv) mature control group v) mature rats exposed to EMF vi) mature rats granted a recovery period of 15 days after exposure termination
|Exposure duration||continuous for two hours/day for 45 days|
|Repetition frequency||217 Hz|
|Distance between exposed object and exposure source||4 cm|
|Chamber||pie cage plexiglas restrainer with twelve slices: one rat/slice; numerous holes for air condition at the top of the restrainer|
|Setup||antenna (height: 15 cm) located in the middle of the pie cage; antenna fixed about 4 cm from the head (due to movements of rats, the distance ranged from 4 to 8 cm)|
|Additional info||band interval of GSM modulator: 1750 - 1850 MHz|
|SAR||0.37 W/kg||-||-||whole body||immature rats|
|SAR||0.49 W/kg||-||-||whole body||mature rats|
|electric field strength||16.62 V/m||mean||calculated||-||± 1,67 V/m (mature rats)|
|electric field strength||16.96 V/m||mean||calculated||-||± 5,39 V/m (immature rats)|
|power||2 W||maximum||-||-||(power of the GSM modulator)|
The data showed significant differences in chromosome aberrations (increase), the micronucleus frequency (increase), mitotic index (decrease) and the ratio of polychromatic erythrocytes (decrease) in bone marrow cells of all exposure groups compared with the control group. Additionally, the cytotoxic and genotoxic damage was more remarkable in immature rats than in mature rats. The 15 days of recovery period was insufficient to compensate the genotoxic damage in immature rats after exposure.