To study whether power frequency magnetic fields induce changes in morphology or protein and/or transcription factor levels (of developmentally regulated transcription factors) in developing mouse embryonic eye lenses.
Three groups of pregnant mice were exposed to magnetic fields of varying intensities from gestation day 0 to 18. The embryonic eye lenses were enucleated on gestation day 18.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
3 h/day for 18 days (from day 0 to 18 of gestation)
|
|
mice were treated in three groups: i) exposure to 1.5 mT ii) exposure to 4.5 mT iii) sham exposure
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 3 h/day for 18 days (from day 0 to 18 of gestation) |
| Exposure source | |
|---|---|
| Setup | pair of Helmholtz coils with 123 turns of 10 mm² copper wire and a radius of 0.25 m each; pregnant mice placed in 4.5 cm x 4.5 cm x 9 cm plastic cages in the center of a 50 cm x 80 cm wooden board between the coils |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.5 mT | - | measured | - | - |
| magnetic flux density | 4.5 mT | - | measured | - | - |
| electric field strength | 27.1 V/m | - | measured | - | - |
| electric field strength | 88.5 V/m | - | measured | - | - |
No effects of 50 Hz magnetic field exposure with intensities of up to 4.5 mT on eye lens development in embryonic mice were detected.
No significant differences were found in eye lens transparency, and the lens epithelial cells from the experimental groups exhibited normal ultrastructure comparable to the control. Furthermore, no significant differences were found among the protein contents and the mRNA expression levels of the transcription factors.
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