Study type: Medical/biological study (experimental study)

Analysis of gene expression in a human-derived glial cell line exposed to 2.45 GHz continuous radiofrequency electromagnetic fields. med./bio.

Published in: J Radiat Res 2011; 52 (2): 185-192

Aim of study (acc. to author)

To study the effects of exposure to radiofrequency electromagnetic fields on gene expression in human fetus-derived SVGp12 cells using a high throughput analysis method.

Background/further details

Three independent experiments were performed under each experimental condition, for a total of nine experimental conditions, consisting of three SAR values at three time points.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 1, 4 or 24 h
  • SAR: 1 W/kg spatial average (at P = 0.11 W)
  • SAR: 5 W/kg spatial average (at P = 0.54 W)
  • SAR: 10 W/kg spatial average (at P = 1.1 W)

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration continuous for 1, 4 or 24 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup 490 mm long cylindrical waveguide terminated by a metallic plate to generate standing waves; culture dish with a diameter of 90 mm placed on the matallic plate inside the waveguide; temperature of the bottom of the culture dish maintained at 36.8 +/- 0.4° C by a Peltier effect device on the metallic plate
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg spatial average measured - at P = 0.11 W
SAR 5 W/kg spatial average measured - at P = 0.54 W
SAR 10 W/kg spatial average measured - at P = 1.1 W

Reference articles

  • Sonoda T et al. (2005): Electromagnetic and thermal dosimetry of a cylindrical waveguide-type in vitro exposure apparatus.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data revealed that 0.28% of about 10,000 genes were altered significantly. 22 genes were further analyzed by RT-PCR to validate the results of microarray, and no significant alterations in gene expression were observed in the exposed cells compared to sham exposed cells.
In conclusion, no evidence was found that exposure to radiofrequency electromagnetic fields affected gene expression in SVGp12 cells.

Study character:

Study funded by

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